Brzyska M, Stege G J, Renkawek K, Bosman G J
Nencki Institute of Experimental Biology, Warsaw, Poland.
Neuroreport. 1998 May 11;9(7):1549-52. doi: 10.1097/00001756-199805110-00056.
The stress proteins hsp25 and alpha B-crystallin are found in increased concentrations in reactive astrocytes of brains undergoing neurodegeneration. In order to characterize this reaction, we investigated the expression of hsp25 and alpha B-crystallin during growth and after stress (heat shock) in glial cells in vitro. In primary rat brain cultures, hsp25 was present in actively dividing astrocytes that were positive for glial fibrillary acidic protein. alpha B-crystallin was found predominantly in oligodendrocytes. Heat shock resulted in increased concentrations of hsp25 and alpha B-crystallin in astrocytes, without any detectable changes in intracellular localization, as detectable with confocal laser microscopy. These results indicate that a neurodegeneration-related increase of the small stress proteins in astrocytes in independent of gliosis per se, and may be a disease-related event.
在经历神经退行性变的大脑中，应激蛋白hsp25和αB-晶状体蛋白在反应性星形胶质细胞中的浓度会升高。为了描述这种反应，我们在体外研究了神经胶质细胞生长期间以及应激（热休克）后hsp25和αB-晶状体蛋白的表达情况。在原代大鼠脑培养物中，hsp25存在于对胶质纤维酸性蛋白呈阳性的活跃分裂的星形胶质细胞中。αB-晶状体蛋白主要存在于少突胶质细胞中。热休克导致星形胶质细胞中hsp25和αB-晶状体蛋白浓度升高，通过共聚焦激光显微镜检测，细胞内定位未发现任何可检测到的变化。这些结果表明，星形胶质细胞中小应激蛋白与神经退行性变相关的增加独立于胶质增生本身，可能是与疾病相关的事件。
