Knowles L M, Milner J A
Nutrition Department, Pennsylvania State University, University Park 16802, USA.
Nutr Cancer. 1998;30(3):169-74. doi: 10.1080/01635589809514659.
The present studies reveal the antiproliferative property of diallyl disulfide (DADS) in cultured human colon tumor cells (HCT-15) relative to its ability to decrease the proportion of cells in the G1 phase and increase the proportion of cells in the G2/M phase. The shift in the proportion of cells blocked in the G2/M phase increased as the concentration and duration of DADS exposure increased. Refeeding DADS-treated cells (50 microM) with complete medium without DADS resulted in a return to normal proliferation rates. Consistent with the G2/M phase arrest, DADS exposure inhibited p34cdc2 kinase activity within four hours of treatment. The maximum depression in p34cdc2 kinase activity (53%) occurred when 25 microM DADS was added to the medium. The present studies suggest that depressed p34cdc2 kinase activity is likely one of the early cellular events that may account for the antiproliferative property of DADS.
目前的研究揭示了二烯丙基二硫化物（DADS）在培养的人结肠肿瘤细胞（HCT-15）中的抗增殖特性，这与其降低G1期细胞比例和增加G2/M期细胞比例的能力有关。随着DADS暴露浓度和持续时间的增加，G2/M期阻滞细胞的比例变化也增加。用不含DADS的完全培养基重新培养经DADS处理的细胞（50 microM），细胞增殖速率恢复正常。与G2/M期阻滞一致，DADS处理4小时内抑制了p34cdc2激酶活性。当向培养基中添加25 microM DADS时，p34cdc2激酶活性的最大抑制率为53%。目前的研究表明，p34cdc2激酶活性降低可能是导致DADS抗增殖特性的早期细胞事件之一。
