Schwarz G
Biophys Struct Mech. 1976 Apr 15;2(1):1-12. doi: 10.1007/BF00535648.
The question is quantitatively examined which general information can be obtained from experimental data of ligand binding to macromolecules if represented by a Scatchard plot. In particular, the curvature as well as the intercepts and slopes at both coordinate axes are analyzed assuming rather general conditions including cooperative behavior (with applications to some simplified cases of interest in practice). The results should provide a basis for attempts to elucidate the binding mechanism of a system encountered in experimental work.
如果用Scatchard图表示，从配体与大分子结合的实验数据中可以获得哪些一般信息。具体而言，在包括协同行为在内的相当一般的条件下（应用于实际中一些感兴趣的简化情况），分析了曲线的曲率以及在两个坐标轴上的截距和斜率。这些结果应为阐明实验工作中遇到的系统的结合机制的尝试提供基础。
