非洲爪蟾TFIIIA基因转录依赖于顺式元件定位和染色质结构。
Xenopus TFIIIA gene transcription is dependent on cis-element positioning and chromatin structure.
作者信息
Pfaff S L, Taylor W L
机构信息
Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee 37232, USA.
出版信息
Mol Cell Biol. 1998 Jul;18(7):3811-8. doi: 10.1128/MCB.18.7.3811.
The Xenopus TFIIIA gene is transcribed very efficiently in oocytes. In addition to a TATA element at -30, we show that from -425 to +7 the TFIIIA gene contains only two positive cis elements centered at -267 (element 1) and -230 (element 2). This arrangement of the cis elements in the TFIIIA gene is striking because these two elements are positioned very close to each other yet separated from the TATA element by approximately 190 nucleotides. We show that the 190-nucleotide spacing between the TATA element and the upstream cis elements (elements 1 and 2) is critical for efficient transcription of the gene in oocytes and that a nucleosome is positioned in this intervening region. This nucleosome may act positively on TFIIIA transcription in oocytes by placing transcription factors bound at elements 1 and 2 in a favorable position relative to the transcription complex at the TATA element.
非洲爪蟾TFIIIA基因在卵母细胞中高效转录。除了位于-30处的TATA元件外，我们还发现，从-425到+7，TFIIIA基因仅包含两个正向顺式元件，分别位于-267（元件1）和-230（元件2）处。TFIIIA基因中顺式元件的这种排列很引人注目，因为这两个元件彼此位置非常靠近，但与TATA元件相隔约190个核苷酸。我们表明，TATA元件与上游顺式元件（元件1和元件2）之间190个核苷酸的间距对于该基因在卵母细胞中的高效转录至关重要，并且在这个间隔区域定位有一个核小体。这个核小体可能通过将结合在元件1和元件2上的转录因子相对于TATA元件处的转录复合物置于有利位置，从而对卵母细胞中TFIIIA的转录产生积极作用。
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