Larsen V F, Spivey M, Holdom R S
Biotechnol Bioeng. 1976 Oct;18(10):1433-43. doi: 10.1002/bit.260181010.
The construction and use of an automated membrane filtration system is described which produces 300 liters per day of sterile medium over at least four months of continuous fermentation. The capacity of the system is limited only by choice of filter type and has applications in continuous pilot plant work or medium-scale batch fermentation, in the bulk supply of communally used laboratory medium, in supplying banks of small-scale continuous fermentors, and for remote control of medium supply in hazardous fermentations (e.g., methane) with great savings in time and materials. Only two sterile storage jars are needed, irrespective of the total demand for the medium.
本文描述了一种自动膜过滤系统的构建与应用，该系统在至少四个月的连续发酵过程中，每天可生产300升无菌培养基。系统的产能仅受过滤类型的选择限制，可应用于连续中试工厂作业或中型分批发酵、公共实验室培养基的批量供应、小型连续发酵罐组的供应，以及危险发酵（如甲烷发酵）中培养基供应的远程控制，可大幅节省时间和材料。无论培养基的总需求量如何，仅需两个无菌储存罐。
