Timoshenko A V, Bovin N V, Shiyan S D, Vakhrushev S Y, André S, Gabius H J
Department of Biophysics, Belarussian State University, Minsk, 220050, Belarus.
Biochemistry (Mosc). 1998 May;63(5):546-50.
The effects of alpha1-acidic glycoprotein (AGP) and its subfractions (glycoforms) with different affinity for concanavalin A on generation of H2O2 by human neutrophils exposed to stimulators of different nature, namely, galactose-specific mistletoe (Viscum album) lectin (VAA), digitonin, and N-formyl-Met-Leu-Phe, a chemotactic peptide (FMLP), were studied. Within the concentration range of 13-500 microgram/ml, AGP and its glycoforms produced dose-dependent inhibition of digitonin-induced cell responses. AGP also inhibited the VAA-induced generation of H2O2; however, this cell response was potentiated by low concentrations (50 microgram/ml) of AGP and AGP-A. FMLP induced the most consistent response of neutrophils, which changed only slightly in the presence of AGP and AGP-B; however, low concentrations of AGP-A inhibited this response. The presence of sialic acid in the terminal position of carbohydrate antennae of AGP is not necessary for its inhibitory effect on human neutrophil respiratory burst because asialo-AGP (250 microgram/ml) inhibited H2O2 generation by cells stimulated with agonists of the NADPH-oxidase system of phagocytes. In contrast to AGP, two other acute phase response proteins displaying a lectin activity (C-reactive protein and serum amyloid P component) within the concentration range of 10-100 microgram/ml produced no significant effect on H2O2 generation by stimulated neutrophils. These data suggest that AGP is an effector molecule responsible for feedback regulation of the functional activity of neutrophils.
研究了与伴刀豆球蛋白A具有不同亲和力的α1-酸性糖蛋白（AGP）及其亚组分（糖型），对暴露于不同性质刺激物（即半乳糖特异性槲寄生（白果槲寄生）凝集素（VAA）、洋地黄皂苷和趋化肽N-甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP））的人中性粒细胞产生H2O2的影响。在13 - 500微克/毫升的浓度范围内，AGP及其糖型对洋地黄皂苷诱导的细胞反应产生剂量依赖性抑制。AGP也抑制VAA诱导的H2O2生成；然而，低浓度（50微克/毫升）的AGP和AGP - A可增强这种细胞反应。FMLP诱导的中性粒细胞反应最为一致，在AGP和AGP - B存在时仅略有变化；然而，低浓度的AGP - A抑制了这种反应。AGP碳水化合物天线末端位置存在唾液酸对于其对人中性粒细胞呼吸爆发的抑制作用并非必需，因为去唾液酸AGP（250微克/毫升）抑制了吞噬细胞NADPH氧化酶系统激动剂刺激的细胞产生H2O2。与AGP相反，另外两种在10 - 100微克/毫升浓度范围内具有凝集素活性的急性期反应蛋白（C反应蛋白和血清淀粉样P成分）对受刺激的中性粒细胞产生H2O2没有显著影响。这些数据表明，AGP是负责中性粒细胞功能活性反馈调节的效应分子。
