Kuroda N, Hosoki S, Nakashima K, Akiyama S, Givens R
School of Pharmaceutical Sciences, Nagasaki University, Japan.
J Biolumin Chemilumin. 1998 Mar-Apr;13(2):101-5. doi: 10.1002/(SICI)1099-1271(199803/04)13:2<101::AID-BIO477>3.0.CO;2-X.
The preparation of a fluorescent labelled oligonucleotide and its photographic detection by peroxyoxalate chemiluminescence (PO-CL) are described. Fluorescent labelling of an oligonucleotide (15-mer) was performed with naphthalene-2,3-dicarboxaldehyde to give an N-substituted 1-cyanobenz[f]isoindole (CBI) derivative (CBI-15-mer). For the photographic detection of CBI-15-mer, the bis(2,6-difluorophenyl) oxalate (DFPO)-dimethyl phthalate (DMP) system was selected to obtain a long-lived CL emission. After optimizing the conditions for the CL reaction, the system was applied to the photographic detection, and as little as 250 fmol per spot of CBI-15-mer on a membrane were detected as a visible spot with an instant photographic film.
本文描述了荧光标记寡核苷酸的制备及其通过过氧草酸酯化学发光（PO-CL）进行的照相检测。用萘-2,3-二甲醛对寡核苷酸（15聚体）进行荧光标记，得到N-取代的1-氰基苯并[f]异吲哚（CBI）衍生物（CBI-15聚体）。为了对CBI-15聚体进行照相检测，选择双（2,6-二氟苯基）草酸酯（DFPO）-邻苯二甲酸二甲酯（DMP）体系以获得长寿命的化学发光发射。在优化化学发光反应条件后，将该体系应用于照相检测，在膜上每点低至250飞摩尔的CBI-15聚体用即时照相胶片可检测为可见斑点。
