Blackwell G J, Flower R J
Br J Pharmacol. 1976 Aug;57(4):589-97. doi: 10.1111/j.1476-5381.1976.tb10389.x.
A novel double-isotope assay for prostaglandin 15-hydroxydehydrogenase (PGDH) is described. 2 The assay is simple, rapid, precise, completely specific for PGDH, and free from artefacts. The results obtained correlate well with a conventional assay. 3 The method is versatile, being applicable to estimations of enzyme activity in cell-free systems as well as perfused organs, and is well suited to the assay of PGDH inhibitors.
本文描述了一种用于前列腺素15-羟基脱氢酶(PGDH)的新型双同位素测定法。该测定法简单、快速、精确,对PGDH具有完全特异性,且无假象。所得结果与传统测定法相关性良好。该方法用途广泛,适用于无细胞系统以及灌注器官中酶活性的测定,非常适合PGDH抑制剂的测定。