一种快速估算前列腺素15-羟基脱氢酶活性的方法及其在药理学中的应用。
A rapid method for the estimation of prostaglandin 15-hydroxydehydrogenase activity and its application to pharmacology.
作者信息
Blackwell G J, Flower R J
出版信息
Br J Pharmacol. 1976 Aug;57(4):589-97. doi: 10.1111/j.1476-5381.1976.tb10389.x.
Abstract
A novel double-isotope assay for prostaglandin 15-hydroxydehydrogenase (PGDH) is described. 2 The assay is simple, rapid, precise, completely specific for PGDH, and free from artefacts. The results obtained correlate well with a conventional assay. 3 The method is versatile, being applicable to estimations of enzyme activity in cell-free systems as well as perfused organs, and is well suited to the assay of PGDH inhibitors.
摘要
本文描述了一种用于前列腺素15-羟基脱氢酶（PGDH）的新型双同位素测定法。该测定法简单、快速、精确，对PGDH具有完全特异性，且无假象。所得结果与传统测定法相关性良好。该方法用途广泛，适用于无细胞系统以及灌注器官中酶活性的测定，非常适合PGDH抑制剂的测定。
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A rapid method for the estimation of prostaglandin 15-hydroxydehydrogenase activity and its application to pharmacology.一种快速估算前列腺素15-羟基脱氢酶活性的方法及其在药理学中的应用。
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