Chau K Y, Lam H Y, Lee K L
Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic University, Kowloon, Hong Kong.
Exp Cell Res. 1998 May 25;241(1):269-72. doi: 10.1006/excr.1998.4052.
The high mobility group (HMG) 1 protein is a highly conserved and ubiquitous chromosomal protein found enriched in active chromatin. In this study, we have investigated the effect of estrogen on the expression of the human high mobility group protein HMG1 gene and found that the HMG1 mRNA level in MCF-7 cells was sharply increased 2.5-fold after 30 min of estrogen treatment. Under continuous estrogen treatment, the HMG1 mRNA level decreased to a 1.5 times that of the basal level at 90 min and remained at this elevated level under estrogen treatment for up to 24 h. These results support the recent finding by Verrier et al. (C.S. Verrier, 1997, Mol. Endocrinol. 11, 1009-1019) that HMG1 promotes the binding of the estrogen receptor to the estrogen response element and further reinforce our believe that HMG1 plays a significant role in estrogen-induced gene expression.
高迁移率族（HMG）1蛋白是一种高度保守且普遍存在的染色体蛋白，在活性染色质中含量丰富。在本研究中，我们研究了雌激素对人高迁移率族蛋白HMG1基因表达的影响，发现雌激素处理30分钟后，MCF - 7细胞中的HMG1 mRNA水平急剧增加2.5倍。在持续雌激素处理下，HMG1 mRNA水平在90分钟时降至基础水平的1.5倍，并在雌激素处理长达24小时的情况下保持在这一升高水平。这些结果支持了Verrier等人最近的发现（C.S. Verrier，1997，《分子内分泌学》11，1009 - 1019），即HMG1促进雌激素受体与雌激素反应元件的结合，并进一步强化了我们的信念，即HMG1在雌激素诱导的基因表达中起重要作用。
