Adam K P, Thiel R, Zapp J, Becker H
Universität des Saarlandes, Saarbrücken, Germany.
Arch Biochem Biophys. 1998 Jun 1;354(1):181-7. doi: 10.1006/abbi.1998.0666.
The incorporation of 13C-labeled glucose into borneol, bornyl acetate, the sesquiterpenes cubebanol and ricciocarpin A, phytol, and stigmasterol has been studied in axenic cultures of the liverworts Ricciocarpos natans and Conocephalum conicum. Quantitative 13C NMR spectroscopic analysis of the resulting labeling patterns showed that the isoprene building blocks of the sesquiterpenes and stigmasterol are built up via the mevalonic acid pathway, whereas the isoprene units of the monoterpenes and the diterpene phytol are exclusively derived from the glyceraldehyde-pyruvate pathway. These results indicate the involvement of both isopentenyl diphosphate biosynthetic pathways in different cellular compartments.
在苔类植物浮苔（Ricciocarpos natans）和地钱（Conocephalum conicum）的无菌培养物中，研究了13C标记的葡萄糖掺入冰片、乙酸龙脑酯、倍半萜荜澄茄醇和水藓萜A、叶绿醇以及豆甾醇的情况。对所得标记模式进行的定量13C NMR光谱分析表明，倍半萜和豆甾醇的异戊二烯结构单元是通过甲羟戊酸途径合成的，而单萜和二萜叶绿醇的异戊二烯单元则完全源自甘油醛-丙酮酸途径。这些结果表明，异戊烯基二磷酸的两条生物合成途径参与了不同的细胞区室。
