Wan J, Wilcock A, Coventry M J
Australian Food Industry Science Centre, Werribee, Victoria, Australia.
J Appl Microbiol. 1998 Feb;84(2):152-8. doi: 10.1046/j.1365-2672.1998.00338.x.
Basil essential oils, including basil sweet linalool (BSL) and basil methyl chavicol (BMC), were screened for antimicrobial activity against a range of Gram-positive and Gram-negative bacteria, yeasts and moulds using an agar well diffusion method. Both essential oils showed antimicrobial activity against most of the micro-organisms examined except Clostridium sporogenes, Flavimonas oryzihabitans, and three species of Pseudomonas. The minimum inhibitory concentration (MIC) of BMC against Aeromonas hydrophila and Pseudomonas fluorescens in TSYE broth (as determined using an indirect impedance method) was 0.125 and 2% (v/v), respectively; the former was not greatly affected by the increase of challenge inoculum from 10(3) to 10(6) cfu ml-1. Results with resting cells demonstrated that BMC was bactericidal to both Aer. hydrophila and Ps. fluorescens. The growth of Aer. hydrophila in filter-sterilized lettuce extract was completely inhibited by 0.1% (v/v) BMC whereas that of Ps. fluorescens was not significantly affected by 1% (v/v) BMC. In addition, the effectiveness of washing fresh lettuce with 0.1 or 1% (v/v) BMC on survival of natural microbial flora was comparable with that effected by 125 ppm chlorine.
使用琼脂孔扩散法，对包括甜罗勒芳樟醇（BSL）和甲基丁香酚（BMC）在内的罗勒精油进行了抗一系列革兰氏阳性菌、革兰氏阴性菌、酵母和霉菌的抗菌活性筛选。两种精油对除产芽孢梭菌、栖稻黄单胞菌和三种假单胞菌属之外的大多数受试微生物均表现出抗菌活性。在胰酪大豆酵母提取物肉汤中（使用间接阻抗法测定），BMC对嗜水气单胞菌和荧光假单胞菌的最低抑菌浓度（MIC）分别为0.125%和2%（v/v）；前者不受挑战接种量从10³增加到10⁶ cfu/ml的显著影响。静息细胞实验结果表明，BMC对嗜水气单胞菌和荧光假单胞菌均具有杀菌作用。0.1%（v/v）的BMC可完全抑制嗜水气单胞菌在经滤膜除菌的生菜提取物中的生长，而1%（v/v）的BMC对荧光假单胞菌的生长没有显著影响。此外，用0.1%或1%（v/v）的BMC清洗新鲜生菜对天然微生物菌群存活的影响与125 ppm氯的效果相当。
