Ringø E, Vadstein O
Foundation of Applied Research, University of Tromsø, Norway.
J Appl Microbiol. 1998 Feb;84(2):227-33. doi: 10.1046/j.1365-2672.1998.00333.x.
Polyclonal antisera made in rabbits against whole washed cells of Vibrio pelagius and Aeromonas caviae were used for detection of these bacterial species in the rearing water and gastrointestinal tract of healthy turbot (Scophthalmus maximus) larvae exposed to V. pelagius and/or Aer. caviae. The results demonstrated that this method is suitable for detection of V. pelagius and Aer. caviae in water samples and larvae at population levels higher than 10(3) ml-1 and 10(3) larva-1. Populations of aerobic heterotrophic bacteria present in the gastrointestinal tract of turbot larvae, estimated using the dilution plate technique, increased from approximately 4 x 10(2) bacteria larva-1 on day 3 post-hatching to approximately 10(5) bacteria fish-1 16 days post-hatching. Sixteen days after hatching, Vibrio spp. accounted for approximately 3 x 10(4) cfu larva-1 exposed to V. pelagius on days 2, 5 and 8 post-hatching. However, only 10(3) of the Vibrio spp. belonged to V. pelagius. When larvae were exposed to Aer. caviae on day 2 post-hatching, the gut microbiota of 5-day old larvae was mainly colonized by Aeromonas spp. (10(4) larva-1), of which 9 x 10(3) belonged to Aer. caviae. Later in the experiment, at the time when high mortality occurred, 9 x 10(5) Aer. caviae were detected. Introduction of V. pelagius to the rearing water seemed to improve larval survival compared with fish exposed to Aer. caviae and with the control group. It was therefore concluded that it is beneficial with regard to larval survival to introduce bacteria (V. pelagius) to the rearing water.
用兔制备的抗费氏弧菌（Vibrio pelagius）和豚鼠气单胞菌（Aeromonas caviae）全菌洗涤细胞的多克隆抗血清，用于检测暴露于费氏弧菌和/或豚鼠气单胞菌的健康大菱鲆（Scophthalmus maximus）幼体养殖水体和胃肠道中的这些细菌种类。结果表明，该方法适用于检测水样和幼体中数量高于10³ ml⁻¹和10³ 幼体⁻¹的费氏弧菌和豚鼠气单胞菌。用稀释平板技术估算，大菱鲆幼体胃肠道中需氧异养细菌数量从孵化后第3天的约4×10² 细菌/幼体增加到孵化后16天的约10⁵ 细菌/鱼。孵化后16天，在孵化后第2、5和8天暴露于费氏弧菌的幼体中，弧菌属细菌数量约为3×10⁴ cfu/幼体。然而，其中只有10³ 的弧菌属细菌属于费氏弧菌。当幼体在孵化后第2天暴露于豚鼠气单胞菌时，5日龄幼体的肠道微生物群主要由气单胞菌属细菌（10⁴ /幼体）定殖，其中9×10³ 属于豚鼠气单胞菌。在实验后期，即高死亡率出现时，检测到9×10⁵ 豚鼠气单胞菌。与暴露于豚鼠气单胞菌的鱼和对照组相比，向养殖水体中引入费氏弧菌似乎提高了幼体存活率。因此得出结论，向养殖水体中引入细菌（费氏弧菌）对幼体存活有益。
