Margolles A, Mayo B, de los Reyes-Gavilán C G
Instituto de Productos Lácteos de Asturias (CSIC), Villaviciosa, Spain.
J Appl Microbiol. 1998 Feb;84(2):255-62. doi: 10.1046/j.1365-2672.1998.00339.x.
Thirty isolates of Listeria monocytogenes and 18 of L. innocua obtained from different short-ripened cheeses manufactured in Asturias (northern Spain), were compared with each other and with reference strains using serotype, phage type and pulsed-field restriction endonuclease digestion profiles analysis of the total DNA. Restriction enzymes ApaI and SmaI defined five clusters in L. monocytogenes (m1 to m5) and two main clusters in L. innocua (i1 and i2). Cluster i2 was further arranged into three subclusters (i2a, i2b and i2c) based on the different Eco52I (XmaIII) and Crf42I (SacII) patterns of its isolates. Clusters of L. innocua were clearly different whereas those of L. monocytogenes were more closely related to each other. In this latter species, serotype 4b isolates (m4 and m5) constituted a more homogeneous group than serogroup I isolates (m1, m2 and m3). Cluster m3 contained two strains of serotype 1/2a whereas m1 and m2 harboured strains of both serotypes, 1/2a and 1/2b. Therefore, the combined use of restriction patterns and serotype may be useful to differentiate L. monocytogenes strains showing identical restriction profiles but differing in serotype. The cheese source of Listeria strains proved that isolates from cluster m1 were repeatedly detected as a contaminant in the same type of cheese. Comparison of L. monocytogenes ApaI profiles showed a genetic proximity of m4 and m5 to the recognized pathogenic strains ATCC 13932 and NCTC 11994, responsible for meningitis cases in other countries. Finally, bacteriophage typing data indicated that m4, the sole phage typable group, had a phage type resembling that of strains causing the Auckland (New Zealand) outbreak of listeriosis in 1969. These data suggest a wide distribution of closely related types which might cause, under several circumstances, sporadic cases of listeriosis.
从西班牙北部阿斯图里亚斯生产的不同短成熟期奶酪中分离出30株单核细胞增生李斯特菌和18株无害李斯特菌，将它们彼此之间以及与参考菌株进行比较，采用血清型、噬菌体分型和总DNA的脉冲场限制性内切酶消化图谱分析。限制性内切酶ApaI和SmaI在单核细胞增生李斯特菌中定义了五个簇（m1至m5），在无害李斯特菌中定义了两个主要簇（i1和i2）。基于i2簇分离株的不同Eco52I（XmaIII）和Crf42I（SacII）图谱，i2簇进一步分为三个亚簇（i2a、i2b和i2c）。无害李斯特菌的簇明显不同，而单核细胞增生李斯特菌的簇彼此之间关系更密切。在后者中，血清型4b分离株（m4和m5）比血清群I分离株（m1、m2和m3）构成了一个更均匀的组。簇m3包含两株血清型1/2a菌株，而m1和m2含有血清型1/2a和1/2b的菌株。因此，限制性图谱和血清型的联合使用可能有助于区分具有相同限制性图谱但血清型不同的单核细胞增生李斯特菌菌株。李斯特菌菌株的奶酪来源证明，来自簇m1的分离株在同类型奶酪中反复被检测为污染物。单核细胞增生李斯特菌ApaI图谱的比较显示，m4和m5与公认的致病菌株ATCC 13932和NCTC 11994在基因上接近，这些菌株在其他国家导致脑膜炎病例。最后，噬菌体分型数据表明，m4是唯一可进行噬菌体分型的组，其噬菌体类型类似于1969年在新西兰奥克兰引起李斯特菌病暴发的菌株。这些数据表明密切相关类型广泛分布，在某些情况下可能导致散发性李斯特菌病病例。
