Dee S A, Joo H S, Park B K, Molitor T W, Bruna G
Department of Clinical and Population Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul 55108, USA.
Vet Rec. 1998 May 23;142(21):569-72. doi: 10.1136/vr.142.21.569.
An attempt was made to eliminate the virus of porcine reproductive and respiratory syndrome from a seedstock farm by using the combined strategies of vaccination and nursery depopulation. The breeding herd was vaccinated with a modified-live virus vaccine; all breeding and lactating adult animals were vaccinated twice, with a 30-day interval between vaccinations. All the sows were vaccinated in this way except for those in the third trimester of gestation (66 to 114 days) which were vaccinated on day 7 of lactation and 30 days later. A serological profiling system was developed to assess when the piglets became infected. Pigs from vaccinated sows were profiled at weekly intervals after weaning, using immunofluorescence tests for the detection of IgM and IgG, a serum neutralising antibody test, and virus isolation. After completion of the protocol, the nursery and finishing sites were monitored for 15 months. Evidence of reinfection in the finishing stage was detected 16 months after depopulation, but not in the nursery or the breeding herd. The source of the virus was not determined, but suspected origins included a lack of biosecurity, aerosol transmission from another infected farm or a persistently infected pig.
为了从一个种猪场清除猪繁殖与呼吸综合征病毒，人们尝试采用疫苗接种和保育舍种群清空的联合策略。繁殖猪群接种了一种弱毒活疫苗；所有繁殖和泌乳成年动物均接种两次，两次接种间隔30天。除妊娠晚期（66至114天）的母猪外，所有母猪均按此方式接种，妊娠晚期母猪在泌乳第7天和30天后接种。开发了一种血清学分析系统来评估仔猪何时感染。断奶后，对接种疫苗母猪所产仔猪每周进行一次分析，采用免疫荧光试验检测IgM和IgG、血清中和抗体试验以及病毒分离。方案完成后，对保育舍和育肥舍进行了15个月的监测。种群清空16个月后，在育肥阶段检测到再次感染的迹象，但在保育舍或繁殖猪群中未检测到。病毒来源未确定，但怀疑的来源包括生物安全措施不足、来自另一个受感染农场的气溶胶传播或持续感染的猪。
