Chubanov V S, Rogov Iu I, Sholukh M V, Konoplia E F
Department of Biochemistry, School of Biology, Belarus State University, Minsk, Belarus.
Vopr Med Khim. 1998 Mar-Apr;44(2):145-50.
In the present work the effects of long-term prenatal gamma-irradiation (0.5 Gy) on the glucagon signalling through adenylyl cyclase have been investigated. In utero gamma-irradiation resulted in the increase of basal and GTP-stimulated adenylyl cyclase activity, whereas, the adenylyl cyclase response to glucagon was essentially reduced. The comparison of kinetic constants estimated from dose-response data for GTP and glucagon suggests that prenatal chronic irradiation prompted (i) the decrease in rate of GTP hydrolysis on Gs-protein; (ii) the reduction of glucagon potency to accelerate the exchange GDP for GTP on Gs-protein.
在本研究中，已对长期产前γ射线照射（0.5 Gy）对通过腺苷酸环化酶的胰高血糖素信号传导的影响进行了研究。子宫内γ射线照射导致基础和GTP刺激的腺苷酸环化酶活性增加，而腺苷酸环化酶对胰高血糖素的反应则基本降低。从GTP和胰高血糖素的剂量反应数据估计的动力学常数比较表明，产前慢性照射促使（i）Gs蛋白上GTP水解速率降低；（ii）胰高血糖素在Gs蛋白上加速GDP与GTP交换的效力降低。
