Porta H, Lizardi P M
Department of Molecular Recognition and Structural Biology, Universidad Nacional Autónoma de México, Cuernavaca, Morelos.
Biotechnology (N Y). 1995 Feb;13(2):161-4. doi: 10.1038/nbt0295-161.
We have constructed an RNA molecule containing a hammerhead ribozyme that is under allosteric control. In the inactive state, the RNA enzyme is unable to cleave a suitable substrate. The formation of the active state of the ribozyme is triggered by a specific interaction with a DNA oligonucleotide effector that is complementary to a single-stranded loop in the RNA enzyme molecule. Other DNA or RNA molecules containing unrelated nucleotide sequences do not function as allosteric effectors. This work demonstrates the feasibility of designing RNA enzymes that are specifically activated in response to an artificially designed molecular recognition event. Such enzymes may have practical applications.
我们构建了一种含有锤头状核酶的RNA分子，该核酶处于变构控制之下。在无活性状态下，RNA酶无法切割合适的底物。核酶活性状态的形成是由与DNA寡核苷酸效应物的特异性相互作用触发的，该效应物与RNA酶分子中的单链环互补。其他含有不相关核苷酸序列的DNA或RNA分子不能作为变构效应物。这项工作证明了设计能响应人工设计的分子识别事件而被特异性激活的RNA酶的可行性。这类酶可能具有实际应用价值。
