单独使用甲醛甲酚与甲醛甲酚加丁香酚对巨噬细胞黏附于塑料表面的影响。
Effects of formocresol alone vs. formocresol with eugenol on macrophage adhesion to plastic surfaces.
作者信息
Segura J J, Jiménez-Rubio A, Calvo J R
机构信息
Department of Dental Pathology and Therapeutics, School of Dentistry and Medical Biochemistry and Molecular Biology, University of Seville, Spain.
出版信息
Pediatr Dent. 1998 May-Jun;20(3):177-80.
PURPOSE
The purpose of this study was to compare the in-vitro effects of a European-based formocresol formulation that incorporates eugenol with formocresol alone on the adhesion of macrophages to plastic surfaces.
METHODS
Macrophages were obtained from Wistar rats. The adherence capacity of macrophages to a plastic surface was determined. Assays were carried out in Eppendorf tubes incubated for 15 min at 37 degrees C in a humidified atmosphere of 5% CO2. The adherence index was calculated.
RESULTS
Results showed that both formocresol/eugenol and formocresol alone significantly decreased the adherence index of macrophages. The formocresol formulation that incorporated eugenol was more potent in inhibiting macrophage adhesion than formocresol alone.
CONCLUSIONS
Taking into account that adherence to a substrate is the first step in the phagocytic process of macrophages and in antigen presentation, both formocresol formulations could inhibit macrophage function and modulate immune and inflammatory responses in dental pulp and periapical tissues.
目的
本研究旨在比较一种含丁香酚的欧洲产甲酚甲醛制剂与单纯甲酚甲醛对巨噬细胞黏附于塑料表面的体外作用。
方法
从Wistar大鼠获取巨噬细胞。测定巨噬细胞对塑料表面的黏附能力。检测在Eppendorf管中于37℃、5%二氧化碳的湿润环境下孵育15分钟后进行。计算黏附指数。
结果
结果显示，甲酚甲醛/丁香酚和单纯甲酚甲醛均显著降低巨噬细胞的黏附指数。含丁香酚的甲酚甲醛制剂在抑制巨噬细胞黏附方面比单纯甲酚甲醛更有效。
结论
鉴于黏附于底物是巨噬细胞吞噬过程和抗原呈递的第一步，两种甲酚甲醛制剂均可抑制巨噬细胞功能，并调节牙髓和根尖周组织中的免疫和炎症反应。
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