Wada I, Satoh M, Takeda T, Nakabayashi T, Honma T, Saitoh H, Takada M, Hirano K
Sapporo National Hospital, Hokkaido, Japan.
Biol Pharm Bull. 1998 May;21(5):535-7. doi: 10.1248/bpb.21.535.
A convenient high-performance liquid chromatography (HPLC) was developed for the rapid assay of granisetron (GRN) in biological fluids, such as serum, urine, and pleural effusion, from cancer patients. Extrelut-1 was used for the solid-phase extraction. HPLC was carried out using a LiChroCART cartridge column packed with Lichrospher 100 CN and a mobile phase consisting of 0.1 M acetate buffer (pH 3.5) and acetonitrile (7:3). A fluorescence detector of 290 nm for excitation and 365 nm for emission was used. The standard curve was linear over the range of 2 to 100 ng/ml of GRN. Assay precision, expressed as a coefficient of variation (C.V.), was in the range of 0.9-5.4% in the within-day assay and 2.5-6.9% in the between-day assay, respectively. GRN was well separated on the HPLC chromatogram from drugs such as etoposide, metclopramide, ondansetron, and domperidone which are often used together with GRN. It was suggested that the present method is useful for the rapid monitoring of GRN in the serum, urine, and pleural effusion of patients undergoing cancer chemotherapy.
开发了一种便捷的高效液相色谱法（HPLC），用于快速测定癌症患者生物体液（如血清、尿液和胸腔积液）中的格拉司琼（GRN）。采用Extrelut-1进行固相萃取。HPLC使用填充有Lichrospher 100 CN的LiChroCART柱以及由0.1 M醋酸盐缓冲液（pH 3.5）和乙腈（7:3）组成的流动相。使用激发波长为290 nm、发射波长为365 nm的荧光检测器。GRN在2至100 ng/ml范围内标准曲线呈线性。日内测定的精密度以变异系数（C.V.）表示，范围为0.9 - 5.4%，日间测定的精密度范围为2.5 - 6.9%。在HPLC色谱图上，GRN与通常与GRN一起使用的药物如依托泊苷、甲氧氯普胺、昂丹司琼和多潘立酮能很好地分离。结果表明，本方法可用于快速监测接受癌症化疗患者血清、尿液和胸腔积液中的GRN。
