Cox M R, Padbury R T, Snelling T L, Schloithe A C, Harvey J R, Toouli J, Saccone G T
Department of Surgery, Flinders University, Flinders Medical Centre, Bedford Park, South Australia, Australia.
Dig Dis Sci. 1998 Jun;43(6):1275-84. doi: 10.1023/a:1018864025835.
The neural distribution and action of gastrin-releasing peptide in the extrahepatic biliary tree of the Australian brush-tailed possum was investigated. Immunohistochemical staining of fixed specimens demonstrated gastrin-releasing peptide-containing nerves throughout the neural plexuses of the gallbladder, sphincter of Oddi, and mucosa of the common bile duct. Gastrin-releasing peptide (5-2000 ng/kg) increased gallbladder tone to a level equivalent to that produced by cholecystokinin octapeptide (160 ng/kg). This action was tetrodotoxin-insensitive. Sphincter of Oddi motility and transsphincteric flow were not altered. Possible mediation of the gallbladder response by gastrin was examined. Gastrin (50-2500 ng/kg) stimulated gastric acid secretion, elevated gallbladder motility to 64% of that produced by gastrin-releasing peptide, and did not alter sphincter of Oddi motility. In conclusion, gastrin-releasing peptide-containing nerves are found in the neural plexus of the possum extrahepatic biliary tree. Gastrin-releasing peptide induces gallbladder contraction in part by a direct action on gallbladder smooth muscle and also via release of gastrin.
研究了胃泌素释放肽在澳大利亚帚尾袋貂肝外胆管树中的神经分布及作用。对固定标本进行免疫组织化学染色显示，在胆囊、奥迪括约肌和胆总管黏膜的神经丛中均存在含胃泌素释放肽的神经。胃泌素释放肽（5 - 2000 ng/kg）可使胆囊张力升高至与八肽胆囊收缩素（160 ng/kg）所产生的水平相当。该作用对河豚毒素不敏感。奥迪括约肌的运动及经括约肌流量未发生改变。研究了胃泌素对胆囊反应的可能介导作用。胃泌素（50 - 2500 ng/kg）刺激胃酸分泌，使胆囊运动增强至胃泌素释放肽所产生水平的64%，且未改变奥迪括约肌的运动。总之，在袋貂肝外胆管树的神经丛中发现了含胃泌素释放肽的神经。胃泌素释放肽部分通过直接作用于胆囊平滑肌以及还通过释放胃泌素诱导胆囊收缩。
