Safe S, Caldwell J
Can J Microbiol. 1976 Aug;22(8):1163-8. doi: 10.1139/m76-169.
Aqueous cell wall and cytoplasm extracts from Mucor rouxii cells grown under acrobic and anaerobic (oxygen-limiting) conditions were obtained. Both of the extracts from the anaerobic and aerobic cells solubilized lanosterol and ergosterol but not to the same extent; ergosterol was complexed about 2-3 times greater than the same concentration of lanosterol. It was also apparent that the relative bindingcapacities of the cell extracts were dependent on the cell growth environment. Two additional fractions were obtained from each extract by (a) methanol precipitation and (b) refluxing in 2% aqueous potassium hydroxide solution followed by methanol precipitation. The binding activities of these precipitates and the corresponding precipitates from yeast extract were compared and the results indicated a range of binding activites which depended on both the extract and the sterol. The data suggested that the in vitro sterol binding of M. rouxii extracts was not due to one particular macromolecule but that a number of compounds were involved in this complexation process.
从在需氧和厌氧（限氧）条件下生长的鲁氏毛霉细胞中获得了细胞壁和细胞质水提取物。厌氧细胞和好氧细胞的提取物都能溶解羊毛甾醇和麦角甾醇，但程度不同；相同浓度下，麦角甾醇的络合量比羊毛甾醇约大2至3倍。细胞提取物的相对结合能力也明显取决于细胞生长环境。通过（a）甲醇沉淀和（b）在2%氢氧化钾水溶液中回流然后甲醇沉淀，从每种提取物中获得了另外两个组分。比较了这些沉淀物和酵母提取物相应沉淀物的结合活性，结果表明结合活性范围取决于提取物和甾醇。数据表明，鲁氏毛霉提取物的体外甾醇结合不是由于一种特定的大分子，而是有多种化合物参与了这种络合过程。
