Royer J C, Moyer D L, Reiwitch S G, Madden M S, Jensen E B, Brown S H, Yonker C C, Johnston J A, Golightly E J, Yoder W T
Novo Nordisk Biotech, Inc., Davis, California 95616, USA.
Biotechnology (N Y). 1995 Dec;13(13):1479-83. doi: 10.1038/nbt1295-1479.
We describe a novel fungal expression system which utilizes the Quorn myco-protein fungus Fusarium graminearum A 3/5. A transformation system was developed for F. graminearum and was used to introduce the coding and regulatory regions of a trypsin gene from Fusarium oxysporum. The protein was efficiently expressed, processed and secreted by the recombinant host strain. In addition, the promoter and terminator of the F. oxysporum trypsin gene have been successfully utilized to drive the expression of a cellulase gene from Scytalidium thermophilum and a lipase gene from Thermomyces lanuginosus in F. graminearum.
我们描述了一种新型真菌表达系统，该系统利用了可食用丝状真菌禾谷镰刀菌A 3/5。我们开发了一种用于禾谷镰刀菌的转化系统，并将其用于导入尖孢镰刀菌胰蛋白酶基因的编码区和调控区。该蛋白在重组宿主菌株中得到了高效表达、加工和分泌。此外，尖孢镰刀菌胰蛋白酶基因的启动子和终止子已成功用于驱动嗜热毁丝霉纤维素酶基因和疏棉状嗜热丝孢菌脂肪酶基因在禾谷镰刀菌中的表达。
