Muramatsu T, Ohmae A, Anzai K
Division of Molecular Cell Biology, College of Pharmacy, Nihon University, Chiba, Japan.
Biochem Biophys Res Commun. 1998 Jun 9;247(1):7-11. doi: 10.1006/bbrc.1998.8657.
Neural BC1 RNA is distributed in dendrites in the form of ribonucleoprotein particles (RNP). Recently, Han et al. reported that testis-brain RNA-binding protein (the mouse homologue of Translin), known to be a translational repressor, links a subset of mRNAs to microtubules (MTs) through binding to their y-,h-sequence elements. We found that similar elements are also present in BC1 RNA. Therefore, it is possible that they also act as cis-elements, together with their transacting proteins, and control the transport of BC1 RNA along dendritic MTs. In this study, we demonstrated that two y-,h-element-binding proteins copurified with BC1 RNP. One was identified as mouse Translin and the other was a 37 kDa protein. This macromolecular assembly may constitute a transport particle for BC1 RNA. It is also possible that BC1 RNP or Translin itself may play a regulatory role(s) in the translation of mRNAs within dendrites.
神经BC1 RNA以核糖核蛋白颗粒（RNP）的形式分布于树突中。最近，韩等人报道，已知作为翻译抑制因子的睾丸-脑RNA结合蛋白（Translin的小鼠同源物）通过与mRNA的γ、η序列元件结合，将一部分mRNA与微管（MT）相连。我们发现BC1 RNA中也存在类似元件。因此，它们有可能与其反式作用蛋白一起作为顺式元件，控制BC1 RNA沿树突状微管的运输。在本研究中，我们证明有两种γ、η元件结合蛋白与BC1 RNP共同纯化。一种被鉴定为小鼠Translin，另一种是37 kDa的蛋白。这种大分子组装可能构成BC1 RNA的运输颗粒。BC1 RNP或Translin本身也有可能在树突内mRNA的翻译中发挥调节作用。
