Quan S, Dabbs E R
Department of Genetics, University of the Witwatersrand, South Africa.
Plasmid. 1993 Jan;29(1):74-9. doi: 10.1006/plas.1993.1010.
A representative of a group of related Rhodococcus arsenic resistance plasmids was characterized, locating the resistance genes and regions influencing host range and controlling copy number. This information, together with identification of antibiotic resistance determinants to replace the arsenic marker was used to construct Rhodococcus-Escherichia coli positive selection shuttle plasmids which, compared with those previously constructed, had half the size, a much higher copy number, and chloramphenicol rather than arsenic as selectable marker.
对一组相关的红球菌属抗砷质粒中的一个代表进行了表征，确定了抗性基因以及影响宿主范围和控制拷贝数的区域。这些信息，连同对可替代砷标记的抗生素抗性决定因素的鉴定，被用于构建红球菌 - 大肠杆菌正向选择穿梭质粒，与先前构建的质粒相比，其大小减半，拷贝数高得多，并且使用氯霉素而非砷作为选择标记。
