Baek B K, Islam M K, Matsuda K
Biosafety Research Institute, Chonbuk National University, Chonju, Korea.
Korean J Parasitol. 1998 Jun;36(2):99-107. doi: 10.3347/kjp.1998.36.2.99.
The present study was performed to check the viability of eggs, filariform larvae and adults of Strongyloides venezuelensis exposed to various conditions for an in vitro maintenance. The eggs in the feces remained viable for about 25 days at 4 degrees C and 15 days at room temperature. However, the isolated eggs in sterile saline lost their viability within 24 hr at 4 degrees C. The eggs in morula stage were very sensitive to air drying and rapidly lost their viability (< or = 12 hr). Filariform larvae survived for a maximum period of 45 days in fecal suspension and 28 days in 0.12% nutrient broth in polyvinyl culture bags maintained at 20 degrees C. On the other hand, those isolated from nutrient broth cultures survived for a maximum period of 32 days in tap water and 22 days in sterile saline at 20 degrees C. The mature adult worms obtained from experimentally infected rats survived maximally for 9 days in serum supplemented (10% rat-serum) 0.12% nutrient broth and 4 days in serum free nutrient broth at 37 degrees C while the culture media were changed at an alternate day. The adult female worms deposited fertile eggs in serum supplemented and serum free nutrient broth cultures, however, the hatched larvae (L1) were not able to develop to the filariform stage in the culture media and found to die within 24 hr of maintenance. The present findings on an in vitro maintenance of different stages of S. venezuelensis may provide useful information for biological and biochemical studies with Strongyloides species.
本研究旨在检测委内瑞拉类圆线虫的虫卵、丝状蚴和成虫在体外维持于各种条件下的活力。粪便中的虫卵在4℃下可存活约25天，在室温下可存活15天。然而，无菌盐水中分离出的虫卵在4℃下24小时内即失去活力。桑葚期虫卵对空气干燥非常敏感，很快失去活力（≤12小时）。丝状蚴在20℃下的粪便悬液中最长存活45天，在0.12%营养肉汤的聚乙烯培养袋中最长存活28天。另一方面，从营养肉汤培养物中分离出的丝状蚴在20℃的自来水中最长存活32天，在无菌盐水中最长存活22天。从实验感染大鼠获得的成熟成虫在补充有10%大鼠血清的0.12%营养肉汤中于37℃下最长存活9天，在无血清营养肉汤中最长存活4天，且每隔一天更换培养基。成年雌虫在补充有血清和无血清的营养肉汤培养物中产出可育虫卵，然而，孵出的幼虫（L1）在培养基中无法发育至丝状蚴阶段，在维持培养24小时内即死亡。委内瑞拉类圆线虫不同阶段体外维持的当前研究结果可能为类圆线虫属的生物学和生物化学研究提供有用信息。
