Rapi S, Ermini A, Bartolini L, Caldini A, Del Genovese A, Miele A R, Buggiani A, Fanelli A
Laboratory Department, Azienda Ospedaliera Careggi, Florence, Italy.
Clin Chem Lab Med. 1998 Apr;36(4):211-4. doi: 10.1515/CCLM.1998.036.
Reticulated platelets are a fraction of newly released circulating elements characterized by a residual amount of RNA. It has been suggested that the reticulated platelet count, providing an estimate of thrombopoiesis in the same way as erythrocyte reticulocyte count is a measure of erythropoiesis, may be useful in the study of thrombocytopenic disorders. Reticulated red cells and platelets can be analyzed by flow cytometry using specific stains for nucleic acids such as Thiazole Orange and Auramine-O. The aim of our work was to perform the simultaneous evaluation of reticulated elements in whole blood using a standard flow cytometer and to correlate the results obtained with a dedicated cytometer. A group of 14 patients with abnormal absolute reticulocyte counts (range 1.1-11%) and a group of 41 patients showing a platelet discrimination error when analyzed with a dedicated flow cytometer (Sysmex R1000) were enrolled. Linear amplification of both scatter and fluorescence was used to perform reticulocyte count. A gate was set on platelet dimensions, and logarithmic amplification of scatter and fluorescence was used to count reticulated platelets. A good correlation was obtained both for results of reticulocyte count (r2 = 0.9825) and for reticulated platelets (r2 = 0.8717) between our method and those using dedicated instruments. These data show that reticulated platelet count may be easily introduced in clinical laboratories that routinely perform reticulocyte count by flow cytometry.
网织血小板是新释放的循环成分的一部分，其特征是含有残留量的RNA。有人提出，网织血小板计数可用于血小板减少症的研究，其作用类似于红细胞网织红细胞计数用于评估红细胞生成，可提供血小板生成的估计值。网织红细胞和血小板可以通过流式细胞术，使用噻唑橙和金胺-O等核酸特异性染色剂进行分析。我们工作的目的是使用标准流式细胞仪对全血中的网织成分进行同步评估，并将所得结果与专用细胞仪的结果进行关联。招募了一组14名绝对网织红细胞计数异常（范围为1.1-11%）的患者和一组41名在用专用流式细胞仪（Sysmex R1000）分析时出现血小板鉴别错误的患者。使用散射光和荧光的线性放大来进行网织红细胞计数。在血小板尺寸上设置一个门，使用散射光和荧光的对数放大来计数网织血小板。我们的方法与使用专用仪器的方法在网织红细胞计数结果（r2 = 0.9825）和网织血小板计数结果（r2 = 0.8717）方面都获得了良好的相关性。这些数据表明，在常规通过流式细胞术进行网织红细胞计数的临床实验室中，可以很容易地引入网织血小板计数。
