培养的岩藻糖苷贮积症成纤维细胞：一种展示氚标记岩藻糖标记物质储存的简单技术。

Cultured fucosidosis fibroblasts: a simple technique demonstrating storage of tritiated-fucose labeled material.

作者信息

Wood S

出版信息

Clin Genet. 1976 Sep;10(3):183-6. doi: 10.1111/j.1399-0004.1976.tb00031.x.

DOI:10.1111/j.1399-0004.1976.tb00031.x

Abstract

Incorporation of tritiated fucose into the acid-soluble and acid-insoluble fractions of cultured fucosidosis and control fibroblast monolayers was determined over a 10-day period. Fucosidosis cells incorporated significantly more label into the acid-soluble, but not the acid-insoluble fraction, than control cells. The ratio of acid-insoluble radioactivity increased lineraly from 1 to 10 days for the fucosidosis cells, indicating the storage of low molecular weight partial degradation products.

摘要

在10天的时间里，测定了氚化岩藻糖掺入培养的岩藻糖苷贮积症和成纤维细胞单层的酸溶性和酸不溶性部分的情况。与对照细胞相比，岩藻糖苷贮积症细胞掺入酸溶性部分的标记物明显更多，但酸不溶性部分则不然。对于岩藻糖苷贮积症细胞，酸不溶性放射性的比例在1至10天内呈线性增加，表明存在低分子量部分降解产物的蓄积。