Pijnenborg R, McLaughlin P J, Vercruysse L, Hanssens M, Johnson P M, Keith J C, Van Assche F A
Department of Obstetrics and Gynaecology, Katholieke Universiteit Leuven, Belgium.
Placenta. 1998 May;19(4):231-9. doi: 10.1016/s0143-4004(98)90054-6.
To identify tumour necrosis factor (TNF)-alpha immunopositive cells, third trimester human placental bed biopsies were selected from nine normotensive control women, 16 severely pre-eclamptic patients and seven patients with pre-existing hypertension with superimposed pre-eclampsia. In addition, five first and early second trimester specimens were included in the study. Immunostaining was performed with a mouse IgG1 monoclonal antibody (J1D9) reactive specifically with human TNF-alpha (1:300 ascitic fluid), using a biotin-streptavidin-peroxidase technique. Variable staining of stromal cells was noted in all biopsies. Specimens of early pregnancy showed marked immunostaining for TNF-alpha on proliferating tips of anchoring villi, invasive interstitial cytotrophoblast (but not the multinuclear giant cells), and endovascular trophoblast invading the spiral arteries. At term, weak staining was found in trophoblast incorporated within spiral artery walls. In biopsies from pre-eclamptic patients, spiral arteries without physiological change showed very little staining except in atherotic vessels where the infiltrated lipophages often showed intense immunolabelling. The marked presence of TNF-alpha in extravillous cytotrophoblast of young specimens is suggestive of a role in early invasion. Immunostaining of foam cells in non-invaded spiral arteries in pre-eclampsia at or near-term indicates a potential role of this cytokine in the development of atherotic lesions.
为了鉴定肿瘤坏死因子（TNF）-α免疫阳性细胞，从9名血压正常的对照女性、16名重度子痫前期患者以及7名合并子痫前期的慢性高血压患者中选取了妊娠晚期人胎盘床活检组织。此外，研究还纳入了5份妊娠早期和孕中期早期的标本。使用生物素-链霉亲和素-过氧化物酶技术，用一种特异性识别人TNF-α的小鼠IgG1单克隆抗体（J1D9，腹水稀释比例为1:300）进行免疫染色。在所有活检组织中均观察到基质细胞的染色情况各异。妊娠早期标本显示，在固定绒毛的增殖顶端、侵入性间质细胞滋养层（而非多核巨细胞）以及侵入螺旋动脉的血管内滋养层中，TNF-α有明显的免疫染色。足月时，在螺旋动脉壁内的滋养层中发现弱阳性染色。在子痫前期患者的活检组织中，未发生生理改变的螺旋动脉染色很浅，除非是动脉粥样硬化血管，其中浸润的噬脂细胞通常显示强烈的免疫标记。年轻标本的绒毛外细胞滋养层中TNF-α的显著存在提示其在早期侵袭中发挥作用。子痫前期足月或接近足月时未受侵袭的螺旋动脉中泡沫细胞的免疫染色表明，这种细胞因子在动脉粥样硬化病变的发展中可能发挥作用。
