肝硬化大鼠动脉组织中的一氧化氮合酶活性

Nitric oxide synthase activity in arterial tissues of cirrhotic rats.

作者信息

Zhang P, Liang K, Lin J, Wang T, Du L

机构信息

Institute of Liver Diseases, Tongji Hospital, Tongji Medical University, Wuhan.

出版信息

J Tongji Med Univ. 1997;17(1):25-7. doi: 10.1007/BF02887997.

DOI:10.1007/BF02887997

PMID:9639780

Abstract

The changes in the activities of constitutive nitric oxide (NO) synthase (cNOS) and inducible NOS (iNOS) were investigated in arterial tissues of CCl4-induced cirrhotic adult SD rats. The aortic tissue homogenate were prepared in normal and cirrhotic rats. NOS activity was measured by conversion of 3H-arginine to 3 H-citrulline. The activities of cNOS and iNOS were calculated in terms of presence or absence of Ca2+. The results showed that activities of total NOS, cNOS and iNOS in arterial tissues were all increased significantly in cirrhotic rats as compared with those in normal controls. There was a significant positive correlation between the activities of total NOS and cGMP content in cirrhotic arterial tissues.

摘要

在四氯化碳诱导的成年肝硬化SD大鼠的动脉组织中，研究了组成型一氧化氮（NO）合酶（cNOS）和诱导型NOS（iNOS）活性的变化。制备正常和肝硬化大鼠的主动脉组织匀浆。通过将3H-精氨酸转化为3H-瓜氨酸来测量NOS活性。根据有无Ca2+计算cNOS和iNOS的活性。结果表明，与正常对照组相比，肝硬化大鼠动脉组织中总NOS、cNOS和iNOS的活性均显著增加。肝硬化动脉组织中总NOS活性与cGMP含量之间存在显著正相关。