Cheng G, Lan H, Sun Z, Zhang K, Du X
Institute of Cardiovascular Disease, Tongji Medical University, Wuhan.
J Tongji Med Univ. 1997;17(1):36-9. doi: 10.1007/BF02888000.
Direct effects of a high-dose aprotinin on the normally perfused hearts and the myocardial protection after ischemia and reperfusion were investigated in an isolated working rat heart model. In trial I, hearts had no ischemia and were perfused with either K-H solution or the K-H solution containing aprotinin (200 KIU/ml) for 55 min. No statistically significant difference was observed in hemodynamics between the two groups. In trial II, hearts were exposed to 150 min period of global ischemia at 15 degrees C with 4 degrees C multidose St. Thomas' II solution (STS). The control group I received normal K-H solution; the group II was treated with the solution with aprotinin added. The group III was similar to the group I and received the STS enriched with aprotinin. On reperfusion, the recovery of hearts in group III was significantly better than those of the group I and II, as reflected by better hemodynamics and myocardial ATP levels and milder myocardial ultrastructural injury. There was no difference between the group I and II. These results suggest that the aprotinin a dose of 200 KIU/ml has no harmful effects on normally perfused hearts and has a marked myocardial protective effect on the prolonged myocardial ischemia when used in cold crystalloid cardioplegia.
在离体工作大鼠心脏模型中，研究了高剂量抑肽酶对正常灌注心脏的直接作用以及对缺血再灌注后心肌的保护作用。在实验I中，心脏无缺血，用K-H溶液或含抑肽酶（200 KIU/ml）的K-H溶液灌注55分钟。两组之间的血流动力学未观察到统计学上的显著差异。在实验II中，心脏在15℃下暴露于150分钟的全心缺血，使用4℃多剂量圣托马斯II液（STS）。对照组I接受正常K-H溶液；组II用添加了抑肽酶的溶液处理。组III与组I相似，接受富含抑肽酶的STS。再灌注时，组III心脏的恢复明显优于组I和组II，表现为更好的血流动力学和心肌ATP水平以及较轻的心肌超微结构损伤。组I和组II之间无差异。这些结果表明，200 KIU/ml剂量的抑肽酶对正常灌注心脏无有害影响，在冷晶体心脏停搏液中使用时对延长的心肌缺血具有显著的心肌保护作用。
