De Silva H D, Van Driel I R, La Gruta N, Toh B H, Gleeson P A
Department of Pathology and Immunology, Monash University Medical School, Prahran, Melbourne, Australia.
Immunology. 1998 Mar;93(3):405-8. doi: 10.1046/j.1365-2567.1998.00436.x.
Murine autoimmune gastritis, induced by neonatal thymectomy, is characterized by a mononuclear infiltrate within the gastric mucosa, loss of parietal and zymogenic cells and circulating autoantibodies to the gastric H/K ATPase. The infiltrate contains both CD4+ and CD8+ T cells. Here we have investigated the roles of CD4+ and CD8+ T cells in the development of gastritis by in vivo treatment with depleting rat anti-CD4 and anti-CD8 monoclonal antibodies. Depletion of CD4+ T cells decreased the incidence of gastric mononuclear infiltrates from 63% (5/8), observed in normal rat immunoglobulin G (IgG)-injected mice, to 8% (1/12) and also abolished the production of antigastric autoantibodies. In contrast, depletion of CD8+ T cells did not reduce the incidence of gastritis. The absence of CD8+ T cells in the infiltrate of the stomach of anti-CD8(+)-treated mice was confirmed by immunocytochemistry. These results argue that neonatal thymectomy-induced autoimmune gastritis is mediated by CD4+ T cells and that CD8+ T cells do not play a significant role in the development of the gastric lesion.
新生期胸腺切除诱导的小鼠自身免疫性胃炎，其特征为胃黏膜内单核细胞浸润、壁细胞和主细胞丧失以及针对胃H/K ATP酶的循环自身抗体。浸润细胞中既有CD4+ T细胞，也有CD8+ T细胞。在此，我们通过用耗竭性大鼠抗CD4和抗CD8单克隆抗体进行体内治疗，研究了CD4+和CD8+ T细胞在胃炎发展中的作用。CD4+ T细胞的耗竭使胃单核细胞浸润的发生率从在注射正常大鼠免疫球蛋白G（IgG）的小鼠中观察到的63%（5/8）降至8%（1/12），并且还消除了抗胃自身抗体的产生。相比之下，CD8+ T细胞的耗竭并未降低胃炎的发生率。通过免疫细胞化学证实，抗CD8(+)处理小鼠胃浸润中不存在CD8+ T细胞。这些结果表明，新生期胸腺切除诱导的自身免疫性胃炎是由CD4+ T细胞介导的，而CD8+ T细胞在胃病变的发展中不发挥重要作用。
