Pasero P, Gasser S M
Swiss Institute for Experimental Cancer Research (ISREC), Epalinges, Switzerland.
Curr Opin Cell Biol. 1998 Jun;10(3):304-10. doi: 10.1016/s0955-0674(98)80004-5.
Current paradigms for the regulation of genomic DNA replication in eukaryotes are derived primarily from cell fusion experiments, yeast genetics, and from in vitro assays in Xenopus egg extracts. Initially, many aspects seemed irreconcilably different among the various organisms and model systems. In the past year, however, divergent approaches have arrived at a consensus on how the cell cycle regulates the initiation of DNA replication. All major players appear to be conserved from yeast to vertebrates, yet the important challenge of reconstituting eukaryotic replication from purified components remains. Three novel in vitro assays that replicate nuclear templates bring us closer to this goal.
目前真核生物基因组DNA复制调控的范式主要源于细胞融合实验、酵母遗传学以及非洲爪蟾卵提取物的体外分析。最初，在各种生物体和模型系统中，许多方面似乎存在着不可调和的差异。然而，在过去的一年里，不同的研究方法就细胞周期如何调控DNA复制的起始达成了共识。从酵母到脊椎动物，所有主要的参与因子似乎都是保守的，然而，用纯化的组分重建真核生物复制这一重要挑战仍然存在。三种能够复制核模板的新型体外分析方法使我们离这个目标更近了一步。
