Lang H, Liu C, Fan E
Beijing Institute Otolaryngology.
Zhonghua Er Bi Yan Hou Ke Za Zhi. 1996;31(6):341-3.
In this in vivo study we investigated the proliferation of crista ampullaris in guinea pigs after Gentamicin intoxication by BrdU immunohistochemical observation and semi-thin sections. The experimental animals were given a single intraperitonial injection of BrdU at a dose of 150 mg/kg body weight before subsequently killed at 1, 3, 7, 14 and 22 day post-treatment (PT). Supporting cell proliferation was evident by BrdU immunoreactivity in the chromatin material of dividing cells at 7 and 14 day PT. The results demonstrated that the crista ampullaris of guinea pigs had repaired by supporting cell mitosis.
在这项体内研究中，我们通过BrdU免疫组织化学观察和半薄切片，研究了庆大霉素中毒后豚鼠壶腹嵴的增殖情况。实验动物在腹腔内单次注射剂量为150mg/kg体重的BrdU，随后在治疗后第1、3、7、14和22天处死。在治疗后第7天和第14天，通过BrdU免疫反应在分裂细胞的染色质物质中可见支持细胞增殖明显。结果表明，豚鼠的壶腹嵴已通过支持细胞有丝分裂得到修复。
