[Bromodeoxyuridine immunohistochemical observation of sensory epithelium repair after ototoxic destruction in the crista ampullaris of guinea pigs].

In this in vivo study we investigated the proliferation of crista ampullaris in guinea pigs after Gentamicin intoxication by BrdU immunohistochemical observation and semi-thin sections. The experimental animals were given a single intraperitonial injection of BrdU at a dose of 150 mg/kg body weight before subsequently killed at 1, 3, 7, 14 and 22 day post-treatment (PT). Supporting cell proliferation was evident by BrdU immunoreactivity in the chromatin material of dividing cells at 7 and 14 day PT. The results demonstrated that the crista ampullaris of guinea pigs had repaired by supporting cell mitosis.