Uezato H, Hagiwara K, Hosokawa A, Maruno M, Nonaka S, Oshiro M, Furuya M, Gomez E A, Hashiguchi Y
Department of Dermatology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
J Dermatol. 1998 May;25(5):290-8. doi: 10.1111/j.1346-8138.1998.tb02400.x.
As a basic study for future diagnosis of cutaneous leishmaniasis, we tried to detect Leishmania parasites representing different species in the subgenera Leishmania and Viannia from subject patients with cutaneous leishmaniasis by using the polymerase chain reaction (PCR) with the subgenus Viannia specific primer. Four out of the 14 specimens revealed an amplified DNA of 70 bp specific for the subgenus Viannia (L. braziliensis complex). No bands were detected in the rest of the specimens belonging to the subgenus Leishmania and unclassified groups. The base sequences of the amplified DNA corresponded with those of the L. (V). braziliensis kinetoplast minicircle. We concluded that PCR using the present primer specific for the subgenus Viannia would be useful in detecting Leishmania parasites in lesions of cutaneous leishmaniasis caused by the L. braziliensis complex.
作为未来皮肤利什曼病诊断的基础研究，我们试图通过使用针对维安亚属的特异性引物进行聚合酶链反应（PCR），从皮肤利什曼病患者中检测代表利什曼原虫属和维安亚属不同物种的利什曼原虫寄生虫。14个标本中有4个显示出针对维安亚属（巴西利什曼原虫复合体）特异性的70 bp扩增DNA。在其余属于利什曼原虫属和未分类组的标本中未检测到条带。扩增DNA的碱基序列与巴西利什曼原虫动质小环的碱基序列一致。我们得出结论，使用目前针对维安亚属的特异性引物进行PCR，将有助于检测由巴西利什曼原虫复合体引起的皮肤利什曼病病变中的利什曼原虫寄生虫。
Diagn Microbiol Infect Dis. 2005-3
Zotero 插件