Passos V M, Fernandes O, Lacerda P A, Volpini A C, Gontijo C M, Degrave W, Romanha A J
Departamento de Clinica Médica, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Acta Trop. 1999 Apr 30;72(3):251-8. doi: 10.1016/s0001-706x(98)00100-4.
Skin biopsies from 53 patients with American cutaneous leishmaniasis (ACL) from the State of Minas Gerais, Brazil, were used for a characterization of the Leishmania parasites. A pair of primers flanking the conserved region of the Leishmania minicircle kDNA was used to obtain amplified DNA via the polymerase chain reaction. The amplified products were subsequently hybridized with Leishmania subgenus-specific radiolabeled probes. Parasites from 49 out of 53 samples (92.5%) were characterized as belonging to the subgenus Viannia and four (7.5%) as belonging to the subgenus Leishmania. Clinical, epidemiological and molecular evidence allow us to conclude that Leishmania (V.) braziliensis and Leishmania (L.) amazonensis are the species present in the patients studied and that L. (V.) braziliensis is the predominant species in the State of Minas Gerais, Brazil.
从巴西米纳斯吉拉斯州53例美洲皮肤利什曼病（ACL）患者身上获取皮肤活检样本，用于利什曼原虫寄生虫的特征分析。一对位于利什曼原虫微小环kDNA保守区域两侧的引物用于通过聚合酶链反应获得扩增DNA。扩增产物随后与利什曼原虫亚属特异性放射性标记探针杂交。53个样本中有49个（92.5%）的寄生虫被鉴定为属于维安亚属，4个（7.5%）属于利什曼亚属。临床、流行病学和分子证据使我们得出结论，在研究的患者中存在巴西利什曼原虫（维安亚属）和亚马逊利什曼原虫（利什曼亚属），并且巴西利什曼原虫（维安亚属）是巴西米纳斯吉拉斯州的主要物种。
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