Iwata A, Masago A, Yamada K
Department of Neurosurgery, Nagoya City University Medical School.
Neurol Med Chir (Tokyo). 1998 May;38(5):257-64; discussion 264-5. doi: 10.2176/nmc.38.257.
The relationship between the expression of basic fibroblast growth factor (bFGF) messenger ribonucleic acid (mRNA) and protein, a potent mitogen for vascular smooth muscle cells in vivo, and administration of the angiotensin-converting enzyme inhibitor cilazapril, which suppresses smooth muscle cells proliferation in denuded arteries, was studied in spontaneously hypertensive rats using the in situ hybridization technique and immunohistochemical study. The effect of cilazapril on neointimal formation through modification of bFGF expression was evaluated using the increased tissue expression of the renin-angiotensin system in spontaneously hypertensive rats. Arterial injury was produced by using balloon catheter denudation in the left carotid artery of rats. The effects were evaluated 2 weeks later. bFGF mRNA and protein were observed only in the endothelial cells of sham-operated rats. bFGF mRNA and protein were observed in both endothelial cells and intimal smooth muscle cells in operated rats receiving only vehicle. Expression of bFGF mRNA and protein was suppressed in both endothelial cells and intimal smooth muscle cells of operated rats receiving cilazapril. These data suggest that cilazapril suppresses smooth muscle cell proliferation through modification of the expression of bFGF mRNA and bFGF protein in addition to other genes.
采用原位杂交技术和免疫组织化学研究方法，在自发性高血压大鼠中，研究了碱性成纤维细胞生长因子（bFGF）信使核糖核酸（mRNA）和蛋白质（一种体内血管平滑肌细胞的有效促有丝分裂原）的表达与血管紧张素转换酶抑制剂西拉普利给药之间的关系。西拉普利可抑制去内皮动脉中平滑肌细胞的增殖。利用自发性高血压大鼠肾素-血管紧张素系统组织表达增加的情况，评估西拉普利通过改变bFGF表达对新生内膜形成的影响。通过球囊导管剥脱大鼠左颈动脉造成动脉损伤。2周后评估效果。仅在假手术大鼠的内皮细胞中观察到bFGF mRNA和蛋白质。在仅接受赋形剂的手术大鼠的内皮细胞和内膜平滑肌细胞中均观察到bFGF mRNA和蛋白质。在接受西拉普利的手术大鼠的内皮细胞和内膜平滑肌细胞中，bFGF mRNA和蛋白质的表达均受到抑制。这些数据表明，西拉普利除了通过其他基因外，还通过改变bFGF mRNA和bFGF蛋白质的表达来抑制平滑肌细胞增殖。
