铜绿假单胞菌分泌弹性蛋白酶及其前肽。
Secretion of elastinolytic enzymes and their propeptides by Pseudomonas aeruginosa.
作者信息
Braun P, de Groot A, Bitter W, Tommassen J
机构信息
Department of Molecular Cell Biology, Utrecht University, The Netherlands.
出版信息
J Bacteriol. 1998 Jul;180(13):3467-9. doi: 10.1128/JB.180.13.3467-3469.1998.
Abstract
Elastase of Pseudomonas aeruginosa is synthesized as a preproenzyme. The signal sequence is cleaved off during transport across the inner membrane and, in the periplasm, proelastase is further processed. We demonstrate that the propeptide and the mature elastase are both secreted but that the propeptide is degraded extracellularly. In addition, reduction of the extracellular proteolytic activity led to the accumulation of unprocessed forms of LasA and LasD in the extracellular medium, which shows that these enzymes are secreted in association with their propeptides. Furthermore, a hitherto undefined protein with homology to a Streptomyces griseus aminopeptidase accumulated under these conditions.
摘要
铜绿假单胞菌的弹性蛋白酶以前体酶原的形式合成。信号序列在跨内膜转运过程中被切除，在周质中，弹性蛋白酶原会进一步加工。我们证明前肽和成熟弹性蛋白酶都会分泌，但前肽会在细胞外被降解。此外，细胞外蛋白水解活性的降低导致未加工形式的LasA和LasD在细胞外培养基中积累，这表明这些酶与其前肽一起分泌。此外，在这些条件下积累了一种与灰色链霉菌氨肽酶具有同源性的迄今未定义的蛋白质。
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本文引用的文献
1
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.空位BLAST和位置特异性迭代BLAST：新一代蛋白质数据库搜索程序。
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402. doi: 10.1093/nar/25.17.3389.
2
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J Bacteriol. 1996 Nov;178(22):6608-17. doi: 10.1128/jb.178.22.6608-6617.1996.
3
Role of the propeptide in folding and secretion of elastase of Pseudomonas aeruginosa.前肽在铜绿假单胞菌弹性蛋白酶折叠与分泌中的作用
Mol Microbiol. 1996 Jan;19(2):297-306. doi: 10.1046/j.1365-2958.1996.381908.x.
4
The elastase propeptide functions as an intramolecular chaperone required for elastase activity and secretion in Pseudomonas aeruginosa.弹性蛋白酶前肽在铜绿假单胞菌中作为弹性蛋白酶活性和分泌所需的分子内伴侣发挥作用。
Mol Microbiol. 1995 Dec;18(5):877-89. doi: 10.1111/j.1365-2958.1995.18050877.x.
5
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Eur J Biochem. 1996 Mar 15;236(3):843-6. doi: 10.1111/j.1432-1033.1996.00843.x.
6
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EMBO J. 1993 Sep;12(9):3357-64. doi: 10.1002/j.1460-2075.1993.tb06009.x.
8
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Mol Microbiol. 1993 Oct;10(2):431-43. doi: 10.1111/j.1365-2958.1993.tb02674.x.
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