Zawadowska B, Müntener M
Department of Cytology and Histology, Jagiellonian University, Kraków, Poland.
Folia Biol (Krakow). 1997;45(3-4):97-101.
Muscle fibre typing is conventionally performed on unfixed frozen sections. Within the particular classes of fibre types mATPase correlates well with the type of myosin heavy chain (MHC). However, investigation of other properties, e.g., parvalbumin (PV) level, cannot be determined on unfixed frozen sections. Determination of PV, which correlates with the relaxation time, is performed immunohistochemically on fixed tissue. By that method a comparison of mATPase activity and the amount of PV within the same muscle fibre is difficult. The present method, combining histochemistry of mATPase and cytochrome c oxidase with parvalbumin immunohistochemistry on consecutive sections, allows the distinction of six fibre types: I, IB, IIA, IIB, IIAB, IIX, and a C fibre population without division into IC and IIC fibres. The advantages and disadvantages of the present method are discussed.
传统上，肌纤维分型是在未固定的冰冻切片上进行的。在特定的纤维类型类别中，线粒体ATP酶（mATPase）与肌球蛋白重链（MHC）的类型密切相关。然而，对其他特性（如小清蛋白（PV）水平）的研究无法在未固定的冰冻切片上进行。与松弛时间相关的PV的测定是在固定组织上通过免疫组织化学方法进行的。通过这种方法，在同一肌纤维内比较mATPase活性和PV含量很困难。本方法将mATPase和细胞色素c氧化酶的组织化学与连续切片上的小清蛋白免疫组织化学相结合，能够区分出六种纤维类型：I型、IB型、IIA型、IIB型、IIAB型、IIX型，以及一个不分为IC型和IIC型的C纤维群体。本文讨论了本方法的优缺点。
