Haskovec C, Kinkor M
Folia Biol (Praha). 1976;22(4):225-34.
A study of various factors influencing the Con A-mediated cell agglutinability failed to reveal any correlation between the agglutinability and the binding capacity of Con A-agglutinable and non-agglutinable human, rabbit, and sheep erythrocytes. Treatment with trypsin and neuraminidase on the one hand and chondroitin sulphate, polyvinylpyrrolidone and polylysine on the other hand made the agglutination of human erythrocytes possible, promoted the agglutination of rabbit erythrocytes, but had almost no effect on sheep erythrocytes, which agglutinated only after treatment with pronase or polylysine. The negligible effects of low temperature, NaN3, cytochalasine B and theophylline on cell agglutinability indicate that neither membrane fluidity, metabolic energy, cAMP, nor the microfilamentous apparatus are likely to play any important role in the Con A-mediated agglutination of erythrocytes. Differences in agglutinability between erythrocytes obtained from different animal species and subjected to different treatments with enzymes and polymers are explained as being due to alterations in the surface charge and the zeta potential.
一项关于影响刀豆球蛋白A介导的细胞凝集性的各种因素的研究未能揭示凝集性与刀豆球蛋白A可凝集和不可凝集的人、兔及绵羊红细胞的结合能力之间存在任何相关性。一方面用胰蛋白酶和神经氨酸酶处理，另一方面用硫酸软骨素、聚乙烯吡咯烷酮和聚赖氨酸处理，使得人红细胞的凝集成为可能，促进了兔红细胞的凝集，但对绵羊红细胞几乎没有影响，绵羊红细胞仅在经链霉蛋白酶或聚赖氨酸处理后才发生凝集。低温、叠氮化钠、细胞松弛素B和茶碱对细胞凝集性的影响可忽略不计，这表明膜流动性、代谢能量、环磷酸腺苷以及微丝装置在刀豆球蛋白A介导的红细胞凝集过程中都不太可能发挥任何重要作用。从不同动物物种获得的红细胞以及经过不同酶和聚合物处理后的红细胞在凝集性上的差异被解释为是由于表面电荷和ζ电位的改变所致。
