Jackwood D J, Sommer S E
Department of Veterinary Preventive Medicine, Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691, USA.
Avian Dis. 1998 Apr-Jun;42(2):321-39.
The genetic heterogeneity of infectious bursal disease virus (IBDV) vaccine strains was compared with IBDV detected in bursa tissue of commercially reared chickens. The vaccine strains tested represented classic viruses from the United States, South Africa, England, and France plus variant viruses from the United States. Bursa tissue samples used for the detection of IBDV from commercially reared chickens were from the United States. Genetic heterogeneity was examined using restriction fragment length polymorphisms (RFLP) of a 743-bp fragment of the VP2 gene that was amplified using reverse transcriptase/polymerase chain reaction (RT/PCR). The RT/PCR products were digested using restriction enzymes BstNI and MboI. On the basis of RFLP profiles, viruses were placed into molecular groups. Thirty-eight vaccine and laboratory strains of IBDV were placed into five molecular groups. Groups 1 and 2 contained variant viruses, groups 3 and 4 contained classic viruses, and group 5 contained Lukert/Edgar strain classic viruses. In contrast to these five molecular groups, 22 molecular groups were observed for 70 IBDV-positive samples from U.S. chicken flocks. Twenty-two of the 70 IBDV-positive samples were placed into molecular groups 1 (2 samples), 2 (18 samples), and 5 (2 samples). Nineteen new RFLP profiles that did not match the five molecular groups observed for vaccine strains were detected. No viruses were observed to have RFLP profiles like molecular groups 3 and 4 observed in the vaccine strains. The genetic heterogeneity was greater among IBDV strains circulating in commercially reared poultry compared with vaccine strains of the virus.
将传染性法氏囊病病毒（IBDV）疫苗株的遗传异质性与在商业饲养鸡的法氏囊组织中检测到的IBDV进行了比较。所测试的疫苗株代表来自美国、南非、英国和法国的经典病毒以及来自美国的变异病毒。用于检测商业饲养鸡中IBDV的法氏囊组织样本来自美国。使用逆转录酶/聚合酶链反应（RT/PCR）扩增VP2基因的743bp片段的限制性片段长度多态性（RFLP）来检查遗传异质性。RT/PCR产物用限制性内切酶BstNI和MboI消化。根据RFLP图谱，将病毒分为分子组。38株IBDV疫苗株和实验室株被分为五个分子组。第1组和第2组包含变异病毒，第3组和第4组包含经典病毒，第5组包含Lukert/Edgar株经典病毒。与这五个分子组形成对比的是，在来自美国鸡群的70个IBDV阳性样本中观察到了22个分子组。70个IBDV阳性样本中的22个被分为分子组1（2个样本）、2（18个样本）和5（2个样本）。检测到19种与疫苗株观察到的五个分子组不匹配的新RFLP图谱。未观察到病毒具有与疫苗株中观察到的分子组3和4相似的RFLP图谱。与该病毒的疫苗株相比，商业饲养家禽中传播的IBDV株之间的遗传异质性更大。
