Sasai K, Lillehoj H S, Hemphill A, Matsuda H, Hanioka Y, Fukata T, Baba E, Arakawa A
Department of Veterinary Medicine, Osaka Prefecture University, Sakai, Japan.
J Parasitol. 1998 Jun;84(3):654-6.
The chicken monoclonal antibody (mAb) 6D12-G10, raised against Eimeria acervulina sporozoites, has previously been shown to recognize the conoid of E. acervulina sporozoites and inhibit sporozoite invasion of lymphocytes in vitro. In indirect immunofluorescent assay, the mAb 6D12-G10 also reacted with merozoites from E. acervulina and identified a 21-kDa merozoite protein on western blots. By confocal laser scanning microscopy, the conoid of sporozoites from 6 different avian Eimeria species (E. brunetti, E. maxima, E. mitis, E. necatrix, E. praecox, and E. tenella) were reactive with 6D12-G10 mAb. Furthermore, the 6D12-G10 mAb also showed cross-reactivity with motile stages of 2 closely related apicomplexans, Neospora, and Toxoplasma. These results indicate that the mAb 6D12-G10 identifies a conserved epitope on the conoid that is important in host cell invasion by the apicomplexan parasites.
抗堆型艾美耳球虫子孢子的鸡单克隆抗体（mAb）6D12-G10，先前已被证明能识别堆型艾美耳球虫子孢子的类锥体，并在体外抑制子孢子对淋巴细胞的侵袭。在间接免疫荧光试验中，mAb 6D12-G10也与堆型艾美耳球虫的裂殖子发生反应，并在蛋白质印迹法中鉴定出一种21 kDa的裂殖子蛋白。通过共聚焦激光扫描显微镜观察，6种不同禽类艾美耳球虫（布氏艾美耳球虫、巨型艾美耳球虫、温和艾美耳球虫、毒害艾美耳球虫、早熟艾美耳球虫和柔嫩艾美耳球虫）子孢子的类锥体与6D12-G10 mAb发生反应。此外，6D12-G10 mAb还与2种密切相关的顶复门原虫（新孢子虫和弓形虫）的活动阶段表现出交叉反应。这些结果表明，mAb 6D12-G10识别了类锥体上一个保守的表位，该表位在顶复门寄生虫入侵宿主细胞过程中很重要。
