来自南美洲的牛分枝杆菌分离株的分子流行病学

Molecular epidemiology of Mycobacterium bovis isolates from South America.

作者信息

Fisanotti J C, Alito A, Bigi F, Latini O, Roxo E, Cicuta E, Zumarraga M J, Cataldi A, Romano M I

机构信息

Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaria, Morón, Buenos Aires, Argentina.

出版信息

Vet Microbiol. 1998 Feb 28;60(2-4):251-7. doi: 10.1016/s0378-1135(98)00150-3.

DOI:10.1016/s0378-1135(98)00150-3

PMID:9646455

Abstract

One hundred seventy-eight isolates of Mycobacterium bovis were subjected to DNA restriction fragment length polymorphism (RFLP) analysis, using the direct repeat element (DR) and the polymorphic GC-rich repeat sequence (PGRS) as probes. By combining the patterns generated by the two repeat DNA elements, 93 different patterns were observed. One hundred-one isolates were grouped in clusters, which include 25 different clusters. One pattern was the most frequently observed, clustering 18.5% of isolates. It was only found in the Center and northeast regions of Argentina and in one isolate from Paraguay. The isolates from Brazil analyzed here presented exclusive patterns (only found in a particular region). The number of exclusive patterns was high in all argentine regions: northeast 78%, center 81%, and Buenos Aires 81%.

摘要

使用直接重复元件（DR）和富含GC的多态性重复序列（PGRS）作为探针，对178株牛分枝杆菌进行了DNA限制性片段长度多态性（RFLP）分析。通过组合两个重复DNA元件产生的图谱，观察到93种不同的图谱。101株分离株被归为簇，其中包括25个不同的簇。一种图谱是最常观察到的，聚集了18.5%的分离株。它仅在阿根廷的中部和东北部地区以及来自巴拉圭的一株分离株中发现。此处分析的来自巴西的分离株呈现出独特的图谱（仅在特定区域发现）。在阿根廷所有地区，独特图谱的数量都很高：东北部为78%，中部为81%，布宜诺斯艾利斯为81%。