Knight M, Redman C W, Linton E A, Sargent I L
Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Oxford.
Br J Obstet Gynaecol. 1998 Jun;105(6):632-40. doi: 10.1111/j.1471-0528.1998.tb10178.x.
To investigate whether syncytiotrophoblast microvilli (STBM) are shed into the maternal circulation in increased amounts in pre-eclamptic pregnancies as a possible cause of maternal vascular endothelial dysfunction.
A time-resolved fluoroimmunoassay was developed to measure STBM levels in peripheral and uterine venous plasma from normal pregnant and pre-eclamptic women. Three colour flow cytometry was used to assess the microparticulate nature of the STBM in pregnancy plasma. The effects of these plasmas on endothelial cell proliferation was compared and a correlation with the levels of STBM detected was sought.
A laboratory investigation using clinical samples obtained from an obstetric practice in a teaching hospital.
Peripheral venous plasma from 20 women with established pre-eclampsia, 20 normal pregnant women matched for age, gestation and parity, and 10 nonpregnant women of reproductive age. Paired uterine and peripheral venous plasma taken at caesarean section from 10 women with pre-eclampsia and 10 unmatched normal pregnant women.
STBM were detected in the plasma of pregnant women by both flow cytometry and time-resolved fluoroimmunoassay. Significantly higher levels of STBM were found in women with established pre-eclampsia (P=0.01). STBM concentrations were higher in uterine venous plasma than in concurrently sampled peripheral venous plasma, confirming their placental origin. A significant correlation was found between the amount of STBM in the plasma and endothelial cell inhibitory activity.
STBM are shed into the maternal circulation (microvillous deportation) and are present in significantly increased amounts in pre-eclamptic women. They may contribute to the endothelial dysfunction underlying the maternal syndrome of pre-eclampsia.
研究子痫前期孕妇合体滋养层微绒毛（STBM）进入母体循环的量是否增加，这可能是导致母体血管内皮功能障碍的原因。
开发了一种时间分辨荧光免疫分析法，用于测量正常孕妇和子痫前期孕妇外周及子宫静脉血浆中的STBM水平。采用三色流式细胞术评估妊娠血浆中STBM的微粒性质。比较这些血浆对内皮细胞增殖的影响，并寻找其与检测到的STBM水平的相关性。
利用从教学医院产科实践中获取的临床样本进行实验室研究。
20例确诊子痫前期的孕妇、20例年龄、孕周和产次匹配的正常孕妇以及10例育龄非孕妇的外周静脉血浆。剖宫产时从10例子痫前期孕妇和10例不匹配的正常孕妇中采集的配对子宫和外周静脉血浆。
通过流式细胞术和时间分辨荧光免疫分析法均在孕妇血浆中检测到STBM。确诊子痫前期的孕妇中STBM水平显著更高（P = 0.01）。子宫静脉血浆中的STBM浓度高于同时采集的外周静脉血浆，证实其来源于胎盘。血浆中STBM的量与内皮细胞抑制活性之间存在显著相关性。
STBM进入母体循环（微绒毛排出），子痫前期女性体内其含量显著增加。它们可能导致子痫前期母体综合征潜在的内皮功能障碍。
