Thompson J, Doxakis E, Piñón L G, Strachan P, Buj-Bello A, Wyatt S, Buchman V L, Davies A M
School of Biomedical Sciences, University of St. Andrews, Bute Medical Buildings, St. Andrews, KY16 9TS, Scotland.
Mol Cell Neurosci. 1998 Jun;11(3):117-26. doi: 10.1006/mcne.1998.0682.
GFRalpha-1, GFRalpha-2, and GFRalpha-3 constitute a family of structurally related, glycosyl-phosphatidylinosital-linked, cell surface proteins, two of which, GFRalpha-1 and GFRalpha-2, are components of the receptor complex for the neurotrophic factors GDNF and neurturin, respectively. By screening an embryonic chicken brain cDNA library with a GFRalpha-1 probe at low stringency, we isolated cDNAs encoding an additional member of the GFRalpha family, GFRalpha-4. The nucleotide sequence predicts a 431-amino-acid secreted protein that is more closely related to GFRalpha-1 and GFRalpha-2 than to GFRalpha-3. GFRalpha-4 mRNA is expressed in distinctive patterns in the brain and several other organs and tissues of the chicken embryo. Our findings extend the family of GFRalpha proteins and provide information about the tissues in which GFRalpha-4 may function during development.
GFRα-1、GFRα-2和GFRα-3构成了一个结构相关、糖基磷脂酰肌醇连接的细胞表面蛋白家族，其中GFRα-1和GFRα-2分别是神经营养因子GDNF和neurturin受体复合物的组成部分。通过用GFRα-1探针在低严谨度下筛选鸡胚脑cDNA文库，我们分离出了编码GFRα家族另一个成员GFRα-4的cDNA。核苷酸序列预测该蛋白为一个431个氨基酸的分泌蛋白，它与GFRα-1和GFRα-2的关系比与GFRα-3的关系更为密切。GFRα-4 mRNA在鸡胚的脑以及其他几个器官和组织中以独特的模式表达。我们的研究结果扩展了GFRα蛋白家族，并提供了有关GFRα-4在发育过程中可能发挥作用的组织的信息。
