Yamamoto M, Tabata Y, Ikada Y
Research Center for Biomedical Engineering, Kyoto University, Japan.
J Biomater Sci Polym Ed. 1998;9(5):439-58. doi: 10.1163/156856298x00550.
Biodegradable hydrogels were prepared from gelatin by glutaraldehyde cross-linking for release matrix of recombinant human bone morphogenetic protein-2 (BMP-2). BMP-2 solution was impregnated into the dried hydrogels to prepare BMP-2-incorporating gelatin hydrogels. In the in vitro study, enhanced retention of BMP-2 was observed from the BMP-2-incorporating gelatin hydrogels after an initial burst of BMP-2 incorporated initially in the hydrogel. Following subcutaneous implantation of (125)I-labeled BMP-2-incorporating gelatin hydrogels in the back of mice, the radioactivity remaining in the hydrogels was measured to estimate the in vivo release profile of BMP-2. It was found that BMP-2 was retained in the hydrogels for longer than 30 days, whereas 99% of BMP-2 injected in the solution form was cleared from the injected site within one day, completely disappearing within 3 days. Ectopic bone formation studies demonstrated that BMP-2-incorporating gelatin hydrogels exhibited a more potent ability for bone induction than solution injection of BMP-2. This finding indicates that enhanced retention of BMP-2 is promotes its ability to induce ectopic bone formation.
通过戊二醛交联由明胶制备可生物降解水凝胶，用于重组人骨形态发生蛋白-2（BMP-2）的释放基质。将BMP-2溶液浸渍到干燥的水凝胶中，制备含BMP-2的明胶水凝胶。在体外研究中，在最初包含在水凝胶中的BMP-2出现初始突释后，观察到含BMP-2的明胶水凝胶中BMP-2的保留增强。在小鼠背部皮下植入（125）I标记的含BMP-2的明胶水凝胶后，测量水凝胶中剩余的放射性，以估计BMP-2的体内释放情况。发现BMP-2在水凝胶中保留超过30天，而以溶液形式注射的BMP-2的99%在一天内从注射部位清除，在3天内完全消失。异位骨形成研究表明，含BMP-2的明胶水凝胶比注射BMP-2溶液表现出更强的骨诱导能力。这一发现表明，BMP-2保留的增强促进了其诱导异位骨形成的能力。
