Wang Y J, Wang X Z, Yang Y H, Qian Y S, Chen W Y, Wang Z S, Fei L S, Chen H W, HE G X, Song Y F
Biology Department, Sichuan University, Chengdu.
Yi Chuan Xue Bao. 1998;25(1):22-7.
Using the cultured giant panda (Ailuropoda melanoleuca) lymphocytes as experimental material, we carried out the terminal marking on the chromosomes which were in replication by adding BrdU (a final concentration: 10 micrograms/ml) about four hours before harvesting the cells. The chromosomes marked by BrdU were proceeded by staining with acridine orange solution (0.05%), irradiated by ultraviolet and counter-stained by Giemsa, we obtained clear chromosomes replication patterns. According to the different replication bands, every chromosome's characteristics in late replication behavior could be identified. In the two X chromosomes of female individual, one X chromosome is obviously much later than the other one. Especially in the large area near centromere on the long arm of late replicating X chromosome. In the male individual, there is also a large area on the long arm of chromosome Y which replicates very late, but the end of long arm of chromosome Y replicates much earlier.
以培养的大熊猫（Ailuropoda melanoleuca）淋巴细胞为实验材料，在收获细胞前约4小时加入BrdU（终浓度：10微克/毫升），对处于复制期的染色体进行末端标记。用吖啶橙溶液（0.05%）对经BrdU标记的染色体进行染色，紫外线照射后再用吉姆萨复染，获得了清晰的染色体复制模式。根据不同的复制带，可以识别每条染色体在复制后期行为的特征。在雌性个体的两条X染色体中，一条X染色体明显比另一条晚得多。特别是在晚复制X染色体长臂靠近着丝粒的大片区域。在雄性个体中，Y染色体长臂上也有一大片区域复制非常晚，但Y染色体长臂末端复制要早得多。
