Saetre S S, Andersen N J, Houe T, Svendsen P, Rehfeld J F, Olsen O, Schaffalitzky de Muckadell O B
Department of Medical Gastroenterology, Odense University Hospital, Denmark.
Acta Physiol Scand. 1998 May;163(1):113-9. doi: 10.1046/j.1365-201x.1998.00349.x.
The importance of physiological plasma levels of secretin in biliary bicarbonate secretion is not known. However, in anaesthetized pigs the substantial hepatic output of bicarbonate into the duodenum in response to low doses of secretin exceeds pancreatic bicarbonate output. The aim was therefore to study the relationship between duodenal acidification, secretin and hepatic biliary bicarbonate output in the conscious pig. Göttingen minipigs (n = 22) were cholecystectomized and the common bile duct catheterized. The biliary bicarbonate secretion in response to intraduodenal HCl, secretin or pentagastrin given intravenously, and to meal was studied. Intraduodenal HCl infusion, secretin and pentagastrin given intravenously augmented hepatic bicarbonate output and plasma secretin concentrations significantly. The secretin response to acidification was sufficient to explain the subsequent increase in biliary bicarbonate secretion. Hepatic bicarbonate secretion and concentrations of CCK and secretin in plasma increased postprandially. Exclusion of bile salts from the duodenum abolished postprandial increase in bile volume and increased release of CCK in fasting and fed pigs whereas secretin release was diminished. The results demonstrate that hepatic bicarbonate secretion is stimulated by endogenous secretin and therefore may have a physiological role in duodenal neutralization.
生理水平的促胰液素在胆汁碳酸氢盐分泌中的重要性尚不清楚。然而，在麻醉猪中，低剂量促胰液素刺激下肝脏向十二指肠大量分泌碳酸氢盐，其分泌量超过胰腺碳酸氢盐的分泌量。因此，本研究旨在探讨清醒猪十二指肠酸化、促胰液素与肝脏胆汁碳酸氢盐分泌之间的关系。选取22只哥廷根小型猪，进行胆囊切除并插入胆总管导管。研究了静脉注射促胰液素、五肽胃泌素，十二指肠内注入盐酸以及进食后胆汁碳酸氢盐的分泌情况。十二指肠内注入盐酸、静脉注射促胰液素和五肽胃泌素均显著增加了肝脏碳酸氢盐的分泌量和血浆促胰液素浓度。促胰液素对酸化的反应足以解释随后胆汁碳酸氢盐分泌的增加。进食后肝脏碳酸氢盐分泌以及血浆中胆囊收缩素（CCK）和促胰液素浓度均升高。十二指肠内排除胆盐可消除进食后胆汁量的增加，并增加禁食和进食猪CCK的释放，而促胰液素的释放减少。结果表明，内源性促胰液素可刺激肝脏碳酸氢盐分泌，因此可能在十二指肠中和过程中发挥生理作用。
