Caramori T, Galizzi A
Dipartimento di Genetica e Microbiologia, Università degli Studi di Pavia, Italy.
Mol Gen Genet. 1998 May;258(4):385-8. doi: 10.1007/s004380050745.
DNA sequences upstream (UP element) of the core promoter (-10, -35 region) of the Bacillus subtilis flagellin gene hag stimulate transcription in vivo and in vitro. We constructed a number of hybrids, placing the UP element of hagp upstream of the core of one SigD-dependent (fliDp) and two SigA-dependent (tmsp, vegp) B. subtilis promoters. The hybrid promoters were fused to a lacZ reporter gene and their activity tested in vivo. The presence of the UP module enhanced transcription at both types of promoters. We conclude that the hagp UP sequence can act as a promoter module independently of the core sequence.
枯草芽孢杆菌鞭毛蛋白基因hag核心启动子（-10、-35区）上游的DNA序列（UP元件）在体内和体外均能刺激转录。我们构建了许多杂种，将hagp的UP元件置于一个SigD依赖性（fliDp）和两个SigA依赖性（tmsp、vegp）枯草芽孢杆菌启动子核心的上游。将杂种启动子与lacZ报告基因融合，并在体内测试其活性。UP模块的存在增强了两种类型启动子的转录。我们得出结论，hagp的UP序列可独立于核心序列作为启动子模块发挥作用。
