Björk-Eriksson T, West C M, Karlsson E, Slevin N J, Davidson S E, James R D, Mercke C
Department of Oncology, Sahlgrenska University Hospital, Gothenburg, Sweden.
Br J Cancer. 1998 Jun;77(12):2371-5. doi: 10.1038/bjc.1998.394.
A study was made of the intrinsic radiosensitivity of 140 biopsy and surgical specimens of malignant head and neck tumours of different histologies. Using a soft-agar clonogenic assay, the material was assessed for the ability to grow in culture (colony-forming efficiency; CFE) and inherent tumour radiosensitivity (surviving fraction at 2 Gy, SF2). The success rate for obtaining growth was 74% (104/140) with a mean CFE of 0.093% (median 0.031) and a range of 0.002-1.3%. SF2 was obtained for 88 of 140 specimens, representing a success rate of 63% with a mean SF2 of 0.48 (median 0.43) and a range of 0.10-1.00. There were no significant differences in radiosensitivity between different sites of the head and neck region. There were no significant relationships between SF2 and disease stage, nodal status, tumour grade, patient age, primary tumour growth pattern and CFE. The results were compared with those for other tumour types previously analysed with the same assay. The distribution of the SF2 values for the head and neck tumours was similar to that for 145 cervix carcinomas and there was no significant difference in mean radiosensitivity between the two tumour types. Also, there was no significant difference in radiosensitivity between head and neck tumours and either breast or colorectal cancers. However, a group of eight lymphomas was significantly more radiosensitive. These results confirm the feasibility of carrying out radiosensitivity measurements using a soft-agar clonogenic assay on head and neck tumours. In addition, the work has shown that radiosensitivity is independent of many clinical parameters and that the mean value is similar to that reported for cervix carcinomas.
对140份不同组织学类型的头颈部恶性肿瘤活检和手术标本的内在放射敏感性进行了研究。采用软琼脂克隆形成试验，评估该材料在培养中的生长能力（集落形成效率；CFE）和内在肿瘤放射敏感性（2 Gy时的存活分数，SF2）。获得生长的成功率为74%（104/140），平均CFE为0.093%（中位数0.031），范围为0.002 - 1.3%。140份标本中有88份获得了SF2，成功率为63%，平均SF2为0.48（中位数0.43），范围为0.10 - 1.00。头颈部不同部位之间的放射敏感性无显著差异。SF2与疾病分期、淋巴结状态、肿瘤分级、患者年龄、原发肿瘤生长模式和CFE之间均无显著相关性。将结果与之前用相同试验分析的其他肿瘤类型的结果进行了比较。头颈部肿瘤的SF2值分布与145例宫颈癌相似，两种肿瘤类型的平均放射敏感性无显著差异。此外，头颈部肿瘤与乳腺癌或结直肠癌之间的放射敏感性也无显著差异。然而，一组8例淋巴瘤的放射敏感性明显更高。这些结果证实了对头颈部肿瘤使用软琼脂克隆形成试验进行放射敏感性测量的可行性。此外，这项工作表明放射敏感性与许多临床参数无关，且平均值与宫颈癌报道的相似。
