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固相cDNA文库构建，一种通用方法。

Solid-phase cDNA library construction, a versatile approach.

作者信息

Roeder T

机构信息

University of Hamburg, Zoological Institute, Department of Neurophysiology, Martin-Luther-King-Platz 3,D-20146 Hamburg, Germany.

出版信息

Nucleic Acids Res. 1998 Jul 15;26(14):3451-2. doi: 10.1093/nar/26.14.3451.

DOI:10.1093/nar/26.14.3451
PMID:9649635
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC147719/
Abstract

A rapid and versatile method for cDNA library construction was developed. It is based on conventional cDNA library synthesis including all enzymatic steps usually required, but is performed on a solid support. The cDNA is immobilised via a biotin residue to streptavidin coupled magnetic beads, which allows rapid and easy to perform changes of buffers and enzymes. Therefore, it combines speed (library construction within a single day) with high quality libraries, making it ideally suited for most purposes.

摘要

开发了一种快速且通用的cDNA文库构建方法。它基于常规的cDNA文库合成，包括通常所需的所有酶促步骤，但在固体支持物上进行。cDNA通过生物素残基固定在与链霉亲和素偶联的磁珠上，这使得缓冲液和酶的更换快速且易于操作。因此，它将速度（一天内构建文库）与高质量文库相结合，使其非常适合大多数用途。

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本文引用的文献

1
High-efficiency cloning of full-length cDNA.全长cDNA的高效克隆
Mol Cell Biol. 1982 Feb;2(2):161-70. doi: 10.1128/mcb.2.2.161-170.1982.
2
A simple and very efficient method for generating cDNA libraries.一种简单且非常有效的生成cDNA文库的方法。
Gene. 1983 Nov;25(2-3):263-9. doi: 10.1016/0378-1119(83)90230-5.
3
Preparation of cDNA and the generation of cDNA libraries: overview.互补DNA（cDNA）的制备及cDNA文库的构建：概述
Methods Enzymol. 1987;152:307-16. doi: 10.1016/0076-6879(87)52035-3.

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