采用两种标准化定量方法（分支DNA法和Amplicor监测法）评估未经治疗的慢性丙型肝炎患者病毒载量的自发波动情况。
Assessment of spontaneous fluctuations of viral load in untreated patients with chronic hepatitis C by two standardized quantitation methods: branched DNA and Amplicor Monitor.
作者信息
Halfon P, Bourlière M, Halimi G, Khiri H, Bertezene P, Portal I, Botta-Fridlund D, Gauthier A P, Jullien M, Feryn J M, Gerolami V, Cartouzou G
机构信息
Alphabio Laboratory, Department of Biochemistry, Hôpital de la Conception, Marseille, France.
出版信息
J Clin Microbiol. 1998 Jul;36(7):2073-5. doi: 10.1128/JCM.36.7.2073-2075.1998.
Abstract
Quantitation of hepatitis C virus (HCV) RNA in serum has been used to predict and monitor the efficacy of interferon therapy in chronic HCV infection. We prospectively studied the fluctuation of viremia by a longitudinal follow-up of HCV RNA levels for 2 months in six untreated patients. Spontaneous fluctuations of HCV RNA ranged from 2.8- to 5.7-fold with branched DNA assay and from 2.9- to 5.6-fold with Monitor. These large spontaneous fluctuations (up to 0.75 log), observed daily, weekly, and monthly, raise doubt about the clinical value of a single assessment of pretherapeutic viremia.
摘要
血清中丙型肝炎病毒（HCV）RNA的定量分析已被用于预测和监测慢性HCV感染患者干扰素治疗的疗效。我们对6例未经治疗的患者进行了前瞻性研究，通过连续2个月纵向随访HCV RNA水平来研究病毒血症的波动情况。采用分支DNA分析法时，HCV RNA的自发波动范围为2.8至5.7倍，采用Monitor检测法时为2.9至5.6倍。每日、每周和每月观察到的这些较大的自发波动（高达0.75对数），让人对治疗前病毒血症单次评估的临床价值产生怀疑。
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